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Outputs (10)

Molecular regulation of axon termination in mechanosensory neurons (2024)
Journal Article
Desbois, M., & Grill, B. (2024). Molecular regulation of axon termination in mechanosensory neurons. Development, 151(17), https://doi.org/10.1242/dev.202945

Spatially and temporally accurate termination of axon outgrowth, a process called axon termination, is required for efficient, precise nervous system construction and wiring. The mechanosensory neurons that sense low-threshold mechanical stimulation... Read More about Molecular regulation of axon termination in mechanosensory neurons.

Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function (2024)
Journal Article
Chang, C., Banerjee, S. L., Soon Park, S., Lei Zhang, X., Cotnoir-White, D., Opperman, K. J., …Kania, A. (2024). Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function. eLife, 12, Article RP89176. https://doi.org/10.7554/eLife.89176.4

Eph receptor tyrosine kinases participate in a variety of normal and pathogenic processes during development and throughout adulthood. This versatility is likely facilitated by the ability of Eph receptors to signal through diverse cellular signallin... Read More about Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function.

Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function (2024)
Journal Article
Chang, C., Banerjee, S. L., Park, S. S., Zhang, X. L., Cotnoir-White, D., Opperman, K. J., …Kania, A. (2024). Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function. eLife, 12, Article RP89176. https://doi.org/10.7554/elife.89176

Eph receptor tyrosine kinases participate in a variety of normal and pathogenic processes during development and throughout adulthood. This versatility is likely facilitated by the ability of Eph receptors to signal through diverse cellular signallin... Read More about Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function.

Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans (2023)
Journal Article
Desbois, M., Pak, J. S., Opperman, K. J., Giles, A. C., & Grill, B. (2023). Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans. STAR Protocols, 4(2), https://doi.org/10.1016/j.xpro.2023.102262

We present an optimized protocol for in vivo affinity purification proteomics and biochemistry using the model organism C. elegans. We describe steps for target tagging, large-scale culture, affinity purification using a cryomill, mass spectrometry a... Read More about Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans.

Loss-of-function variants in MYCBP2 cause neurobehavioural phenotypes and corpus callosum defects (2022)
Journal Article
AlAbdi, L., Desbois, M., Rusnac, D., Sulaiman, R. A., Rosenfeld, J. A., Lalani, S., …Alkuraya, F. S. (2023). Loss-of-function variants in MYCBP2 cause neurobehavioural phenotypes and corpus callosum defects. Brain, 146(4), https://doi.org/10.1093/brain/awac364

The corpus callosum is a bundle of axon fibres that connects the two hemispheres of the brain. Neurodevelopmental disorders that feature dysgenesis of the corpus callosum as a core phenotype offer a valuable window into pathology derived from abnorma... Read More about Loss-of-function variants in MYCBP2 cause neurobehavioural phenotypes and corpus callosum defects.

Ubiquitin ligase activity inhibits Cdk5 to control axon termination (2022)
Journal Article
Desbois, M., J. Opperman, K., Amezquita, J., Gaglio, G., Crawley, O., & Grill, B. (2022). Ubiquitin ligase activity inhibits Cdk5 to control axon termination. PLOS genetics, 18(4), Article e1010152. https://doi.org/10.1371/journal.pgen.1010152

The Cdk5 kinase plays prominent roles in nervous system development, plasticity, behavior and disease. It also has important, non-neuronal functions in cancer, the immune system and insulin secretion. At present, we do not fully understand negative r... Read More about Ubiquitin ligase activity inhibits Cdk5 to control axon termination.

Autophagy is inhibited by ubiquitin ligase activity in the nervous system (2019)
Journal Article
Crawley, O., Opperman, K. J., Desbois, M., Adrados, I., Borgen, M. A., Giles, A. C., …Grill, B. (2019). Autophagy is inhibited by ubiquitin ligase activity in the nervous system. Nature Communications, 10, Article 5017. https://doi.org/10.1038/s41467-019-12804-3

Autophagy is an intracellular catabolic process prominent in starvation, aging and disease. Neuronal autophagy is particularly important, as it affects the development and function of the nervous system, and is heavily implicated in neurodegenerative... Read More about Autophagy is inhibited by ubiquitin ligase activity in the nervous system.

A complex containing the O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 regulates GABA neuron function (2019)
Journal Article
Giles, A. C., Desbois, M., Opperman, K. J., Tavora, R., Maroni, M. J., & Grill, B. (2019). A complex containing the O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 regulates GABA neuron function. Journal of biological chemistry, 294(17), 6843-6856. https://doi.org/10.1074/jbc.ra119.007406

Inhibitory GABAergic transmission is required for proper circuit function in the nervous system. However, our understanding of molecular mechanisms that preferentially influence GABAergic transmission, particularly presynaptic mechanisms, remains lim... Read More about A complex containing the O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 regulates GABA neuron function.

PAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2 (2018)
Journal Article
Desbois, M., Crawley, O., Evans, P. R., Baker, S. T., Masuho, I., Yasuda, R., & Grill, B. (2018). PAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2. Journal of biological chemistry, 293(36), 13897-13909. https://doi.org/10.1074/jbc.ra118.002176

PHR (PAM/Highwire/RPM-1) proteins are conserved RING E3 ubiquitin ligases that function in developmental processes, such as axon termination and synapse formation, as well as axon degeneration. At present, our understanding of how PHR proteins form u... Read More about PAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2.

A MIG-15/JNK-1 MAP kinase cascade opposes RPM-1 signaling in synapse formation and learning (2017)
Journal Article
Crawley, O., Giles, A. C., Desbois, M., Kashyap, S., Birnbaum, R., & Grill, B. (2017). A MIG-15/JNK-1 MAP kinase cascade opposes RPM-1 signaling in synapse formation and learning. PLOS genetics, 13(12), Article e1007095. https://doi.org/10.1371/journal.pgen.1007095

The Pam/Highwire/RPM-1 (PHR) proteins are conserved intracellular signaling hubs that regulate synapse formation and axon termination. The C. elegans PHR protein, called RPM-1, acts as a ubiquitin ligase to inhibit the DLK-1 and MLK-1 MAP kinase path... Read More about A MIG-15/JNK-1 MAP kinase cascade opposes RPM-1 signaling in synapse formation and learning.