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Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line.

Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line. Thumbnail


Abstract

Aluminium-based adjuvants (ABA) are the predominant adjuvants used in human vaccinations. While a consensus is yet to be reached on the aetiology of the biological activities of ABA several studies have identified shape, crystallinity and size as critical factors affecting their adjuvanticity. In spite of recent advances, the fate of ABA following their administration remains unclear. Few if any studies have demonstrated the unequivocal presence of intracellular ABA. Herein we demonstrate for the first time the unequivocal identification of ABA within a monocytic T helper 1 (THP-1) cell line, using lumogallion as a fluorescent molecular probe for aluminium. Use of these new methods revealed that particulate ABA was only found in the cell cytoplasm. Transmission electron microscopy revealed that ABA were contained within vesicle-like structures of approximately 0.5-1 µm in diameter.

Citation

(2014). Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line. Scientific reports, 6287 - ?. https://doi.org/10.1038/srep06287

Acceptance Date Aug 15, 2014
Publication Date Sep 5, 2014
Journal Scientific Reports
Print ISSN 2045-2322
Publisher Nature Publishing Group
Pages 6287 - ?
DOI https://doi.org/10.1038/srep06287
Keywords Cell biology, Fluorescence imaging
Publisher URL http://www.nature.com/articles/srep06287

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