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Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.

Chapman, V; Markides, H; Sagar, DR; Xu, L; Kay, A; Burston, JJ; Mapp, P; Morris, RH; Kehoe, O; El Haj, AJ

Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis. Thumbnail


Authors

V Chapman

H Markides

DR Sagar

L Xu

A Kay

JJ Burston

P Mapp

RH Morris

AJ El Haj



Abstract

Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA. Methods: Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5?×?106late passage MSCs labelled with 10?µg/ml SiMAG, 1.5?×?106late passage mesenchymal stem cells, the steroid Kenalog (200?µg/20?µL), 1.5?×?106early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint. Results: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology. Conclusion: Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.

Acceptance Date Sep 7, 2017
Publication Date Dec 24, 2017
Journal Stem Cells International
Print ISSN 1687-966X
Publisher Hindawi
Pages 2905104 - ?
DOI https://doi.org/10.1155/2017/2905104
Publisher URL https://www.hindawi.com/journals/sci/2017/2905104/

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