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Phanta: a non-fluorescent photochromic acceptor for pcFRET.

Phanta: a non-fluorescent photochromic acceptor for pcFRET. Thumbnail


Abstract

We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.

Citation

(2013). Phanta: a non-fluorescent photochromic acceptor for pcFRET. PloS one, e75835 - ?. https://doi.org/10.1371/journal.pone.0075835

Acceptance Date Aug 22, 2013
Publication Date Sep 30, 2013
Journal PloS one
Print ISSN 1932-6203
Publisher Public Library of Science
Pages e75835 - ?
DOI https://doi.org/10.1371/journal.pone.0075835
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075835

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