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Identification of a Glass Substrate to Study Cells Using Fourier Transform Infrared Spectroscopy: Are We Closer to Spectral Pathology?

Rutter, AV; Crees, J; Wright, H; Raseta, M; van Pittius, DG; Roach, P; Sulé-Suso, J


J Crees

H Wright

M Raseta

DG van Pittius

P Roach


The rising incidence of cancer worldwide is causing an increase in the workload in pathology departments. This, coupled with advanced analysis methodologies, supports a developing need for techniques that could identify the presence of cancer cells in cytology and tissue samples in an objective, fast, and automated way. Fourier transform infrared (FT-IR) microspectroscopy can identify cancer cells in such samples objectively. Thus, it has the potential to become another tool to help pathologists in their daily work. However, one of the main drawbacks is the use of glass substrates by pathologists. Glass absorbs IR radiation, removing important mid-IR spectral data in the fingerprint region (1800?cm-1 to 900?cm-1). In this work, we hypothesized that, using glass coverslips of differing compositions, some regions within the fingerprint area could still be analyzed. We studied three different types of cells (peripheral blood mononuclear cells, a leukemia cell line, and a lung cancer cell line) and lymph node tissue placed on four different types of glass coverslips. The data presented here show that depending of the type of glass substrate used, information within the fingerprint region down to 1350?cm-1 can be obtained. Furthermore, using principal component analysis, separation between the different cell lines was possible using both the lipid region and the fingerprint region between 1800?cm-1 and 1350?cm-1. This work represents a further step towards the application of FT-IR microspectroscopy in histopathology departments.

Journal Article Type Article
Acceptance Date Aug 19, 2019
Online Publication Date Sep 13, 2019
Publication Date Sep 13, 2019
Journal Applied Spectroscopy
Print ISSN 0003-7028
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
Volume 74
Issue 2
Pages 178-186
Keywords FT-IR microspectroscopy, Fourier transform infrared microspectroscopy, glass coverslips, leukemia, lung cancer, peripheral blood mononuclear cells
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