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Divya Chari's Outputs (74)

Neurosurgical grade biomaterial, DuraGenTM, offers a promising matrix for protected delivery of neural stem cells in clinical cell therapies (2019)
Journal Article
Finch, L., Adams, C., Sen, J., Tickle, J., Tzerakis, N., & Chari, D. M. (2019). Neurosurgical grade biomaterial, DuraGenTM, offers a promising matrix for protected delivery of neural stem cells in clinical cell therapies. Future Healthcare Journal, 6(Suppl 1), 76-76. https://doi.org/10.7861/futurehosp.6-1-s76

Aims
Transplantation of neural stem cells (NSCs) into sites of neurological injury is being investigated in clinical trials around the world. These important self-renewing and multipotent precursors can generate the major central nervous system cell... Read More about Neurosurgical grade biomaterial, DuraGenTM, offers a promising matrix for protected delivery of neural stem cells in clinical cell therapies.

Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices (2019)
Journal Article
Adams, C., Delaney, A., Carwardine, D., Tickle, J., Granger, N., & Chari, D. (2019). Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices. Macromolecular Bioscience, 19, e1800389. https://doi.org/10.1002/mabi.201800389

A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due t... Read More about Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices.

Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy (2018)
Journal Article
Tickle, J. A., Poptani, H., Taylor, A., & Chari, D. M. (2018). Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy. Nanomedicine, 13(11), 1333-1348. https://doi.org/10.2217/nnm-2017-0347

Aim: To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI. Materials & methods: Astrocytes were exogenously labe... Read More about Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy.

A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells. (2018)
Journal Article
Al-Mayyahi, R. S., Sterio, L. D., Connolly, J. B., Adams, C., Al-Tumah, W. A., Sen, J., …Chari, D. (2018). A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells. Molecular and Cellular Neuroscience, 86, 30-40. https://doi.org/10.1016/j.mcn.2017.11.006

Corticosteroids (CSs) are widely used clinically, for example in pediatric respiratory distress syndrome, and immunosuppression to prevent rejection of stem cell transplant populations in neural cell therapy. However, such treatment can be associated... Read More about A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells..

Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles (2017)
Journal Article
Evans, M., Shakli, A., Jenkins, S., & Chari, D. (2017). Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles. Nano Research, 2881-2890. https://doi.org/10.1007/s12274-017-1496-4

The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction m... Read More about Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.

A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells. (2017)
Journal Article
Delaney, A., Adams, C., Fernandes, A., Al-Shakli, A., Sen, J., Carwardine, D., …Chari, D. (2017). A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells. Nanoscale, 9, 8560--8566. https://doi.org/10.1039/c7nr00811b

Olfactory ensheathing cells (OECs) promote axonal regeneration and improve locomotor function when transplanted into the injured spinal cord. A recent clinical trial demonstrated improved motor function in domestic dogs with spinal injury following a... Read More about A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells..

Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes. (2017)
Book Chapter
Pickard, M., Adams, C., & Chari, D. (2017). Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes. In Current Protocols in Stem Cell Biology (2D.19.1 - 2D.19.16). https://doi.org/10.1002/9780470151808

Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs)... Read More about Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes..

Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology. (2016)
Journal Article
Adams, C., Dickson, A., Kuiper, J., & Chari, D. (2016). Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology. Nanoscale, 8, 17869-17880. https://doi.org/10.1039/c6nr05244d

Tissue engineering studies are witnessing a major paradigm shift to cell culture on biomimetic materials that replicate native tissue features from which the cells are derived. Few studies have been performed in this regard for neural cells, particul... Read More about Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology..

Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology (2016)
Journal Article
Fernandes, A. R., & Chari, D. M. (2016). Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology. Journal of Controlled Release, 300-310. https://doi.org/10.1016/j.jconrel.2016.06.039

Both neurotrophin-based therapy and neural stem cell (NSC)-based strategies have progressed to clinical trials for treatment of neurological diseases and injuries. Brain-derived neurotrophic factor (BDNF) in particular can confer neuroprotective and... Read More about Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology.

Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy (2016)
Journal Article
Chari, & Fernandes, A. R. (2016). Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy. Journal of Controlled Release, 289-299. https://doi.org/10.1016/j.jconrel.2016.06.024

Genetically engineered neural stem cell (NSC) transplant populations offer key benefits in regenerative neurology, for release of therapeutic biomolecules in ex vivo gene therapy. NSCs are ‘hard-to-transfect’ but amenable to ‘magnetofection’. Despite... Read More about Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy.

Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake (2016)
Journal Article
Weightman, A. P., Jenkins, S. I., & Chari, D. M. (2016). Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake. Nano Research, 9(8), 2384-2397. https://doi.org/10.1007/s12274-016-1125-7

Development of nanoparticle (NP) based therapies to promote regeneration in sites of central nervous system (CNS; i.e. brain and spinal cord) pathology relies critically on the availability of experimental models that offer biologically valid predict... Read More about Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake.

Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells (2016)
Journal Article
Chari, & Adams. (2016). Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells. Advanced healthcare materials, 841-849. https://doi.org/10.1002/adhm.201500885

Genetic modification of cell transplant populations and cell tracking ability are key underpinnings for effective cell therapies. Current strategies to achieve these goals utilize methods which are unsuitable for clinical translation because of relat... Read More about Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells.

Development of Multifunctional Magnetic Nanoparticles for Genetic Engineering and Tracking of Neural Stem Cells (2016)
Journal Article
Adams, C., Israel, L. L., Ostrovsky, S., Taylor, A., Poptani, H., Lellouche, J., & Chari, D. (2016). Development of Multifunctional Magnetic Nanoparticles for Genetic Engineering and Tracking of Neural Stem Cells. Advanced healthcare materials, 5(7), 841-849. https://doi.org/10.1002/adhm.201500885

Genetic modification of cell transplant populations and cell tracking ability are key underpinnings for effective cell therapies. Current strategies to achieve these goals utilize methods which are unsuitable for clinical translation because of relat... Read More about Development of Multifunctional Magnetic Nanoparticles for Genetic Engineering and Tracking of Neural Stem Cells.

Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. (2016)
Journal Article
Tickle, J., Jenkins, S., Polyak, B., Pickard, M., & Chari, D. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 345 - 358. https://doi.org/10.2217/nnm.15.202

AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of var... Read More about Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance..

'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics (2016)
Journal Article
Jenkins, S., Weinberg, D., Al-Shakli, A., Fernandes, A., Yiu, H., Telling, N., …Chari, D. (2016). 'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics. Journal of Controlled Release, 224, 136-145. https://doi.org/10.1016/j.jconrel.2016.01.013

Surface engineering to control cell behavior is of high interest across the chemical engineering, drug delivery and biomaterial communities. Defined chemical strategies are necessary to tailor nanoscale protein interactions/adsorption, enabling contr... Read More about 'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics.

Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations. (2015)
Journal Article
Adams, C., Rai, A., Sneddon, G., Yiu, H., Polyak, B., & Chari, D. (2015). Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations. Nanomedicine, 11, 19--29. https://doi.org/10.1016/j.nano.2014.07.001

Safe and efficient delivery of therapeutic cells to sites of injury/disease in the central nervous system is a key goal for the translation of clinical cell transplantation therapies. Recently, 'magnetic cell localization strategies' have emerged as... Read More about Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations..