Skip to main content

Research Repository

Advanced Search

Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis (2022)
Journal Article
Cosgrove, Miller, & Dolan. (2022). Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis. JACS Au, 47-61. https://doi.org/10.1021/jacsau.2c00529

While the field of biocatalysis has bloomed over the past 20–30 years, advances in the understanding and improvement of carbohydrate-active enzymes, in particular, the sugar nucleotides involved in glycan building block biosynthesis, have progressed... Read More about Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.

Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase. (2022)
Journal Article
Ahmadipour, S., Wahart, A., Dolan, J., Beswick, L., Hawes, C., Field, R., & Miller, G. (2022). Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase. Beilstein Journal of Organic Chemistry, 18, 1379 - 1384. https://doi.org/10.3762/bjoc.18.142

Sufferers of cystic fibrosis are at significant risk of contracting chronic bacterial lung infections. The dominant pathogen in these cases is mucoid Pseudomonas aeruginosa. Such infections are characterised by overproduction of the exopolysaccharide... Read More about Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase..

Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions (2022)
Journal Article
P. Dolan, J., Machin, D. C., Dedola, S., Field2, R. A., Webb, M. E., & Bruce Turnbull, W. (2022). Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions. Frontiers in Chemistry, https://doi.org/10.3389/fchem.2022.958272

<jats:p>The chemoenzymatic synthesis of a series of dual N- and C-terminal–functionalized cholera toxin B subunit (CTB) glycoconjugates is described. Mucin 1 peptides bearing different levels of Tn antigen glycosylation [MUC1(Tn)] were prepared <jats... Read More about Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions.

Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins (2022)
Journal Article
McBerney, R., Dolan, J., E. Cawood, E., E. Webb, M., & Bruce Turnbull, W. (2022). Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins. JACS Au, https://doi.org/10.1021/jacsau.2c00312

Post-translational glycosylation of proteins results in complex mixtures of heterogeneous protein glycoforms. Glycoproteins have many potential applications from fundamental studies of glycobiology to potential therapeutics, but generating homogeneou... Read More about Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins.

Chemical and enzymatic synthesis of the alginate sugar nucleotide building block: GDP-d-mannuronic acid (2019)
Journal Article
Beswick, L., Ahmadipour, S., Dolan, J. P., Rejzek, M., Field, R. A., & Miller, G. J. (2019). Chemical and enzymatic synthesis of the alginate sugar nucleotide building block: GDP-d-mannuronic acid. Carbohydrate Research, 485(1), 107819 -107819. https://doi.org/10.1016/j.carres.2019.107819

Highlights

Sugarnucleotide synthesis.

Chemical and enzymatic access to GDP-d-ManA.

Evaluation of protected and free uronate 1-phosphates for pyrophosphorylation.

Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system (2017)
Journal Article
O'Brien, M., Cooper, D., & Dolan, J. (2017). Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system. Tetrahedron Letters, 829 - 834. https://doi.org/10.1016/j.tetlet.2017.01.029

Abstract A dynamic computer-vision control system incorporating open-source software technologies (Python, OpenCV) was used to automate a gravity based in-line liquid-liquid extraction during the iodination of enaminones in continuous flow. The syste... Read More about Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system.