Detection of regional DNA methylation using DNA-graphene affinity interactions

Haque, Md. Hakimul; Gopalan, Vinod; Yadav, Sharda; Islam, Md. Nazmul; Eftekhari, Ehsan; Li, Qin; Carrascosa, Laura G.; Nguyen, Nam-Trung; Lam, Alfred K.; Shiddiky, Muhammad J.A.

Abstract

We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.

Citation

Haque, M. H., Gopalan, V., Yadav, S., Islam, M. N., Eftekhari, E., Li, Q., Carrascosa, L. G., Nguyen, N.-T., Lam, A. K., & Shiddiky, M. J. (2016). Detection of regional DNA methylation using DNA-graphene affinity interactions. Biosensors and Bioelectronics, 87, 615-621. https://doi.org/10.1016/j.bios.2016.09.016

• Journal Article Type: Article
• Acceptance Date: Sep 4, 2016
• Online Publication Date: Sep 5, 2016
• Publication Date: Sep 5, 2016
• Deposit Date: Jun 25, 2025
• Journal: Biosensors and Bioelectronics
• Print ISSN: 0956-5663
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 87
• Pages: 615-621
• DOI: https://doi.org/10.1016/j.bios.2016.09.016
• Keywords: DNA methylation; Regional methylation; Electrochemical detection; Graphene-DNA base interaction; Disposable screen-printed electrode
• Public URL: https://keele-repository.worktribe.com/output/1054088
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0956566316308879?via%3Dihub