Clinical and Cellular Predictors of Outcomes in Autologous Conditioned Plasma Therapy for Knee Osteoarthritis: A Prospective Cohort Study

Selim, Amr; Lan, Tian; Hulme, Charlotte; Williams, Mike; Perry, Jade; Gallacher, Pete; Jermin, Paul; Wright, Karina

Abstract

Introduction Autologous conditioned plasma (ACP) is a single-spin, leukocyte-poor platelet-rich plasma (PRP) that provides a plasma with a platelet concentration 2 to 3 times the blood platelet concentration. The objective of this study was to investigate the clinical effectiveness of ACP intra-articular injection in patients with knee osteoarthritis (OA) and to identify any demographic, disease-associated, or biological predictors of outcome. Methods A prospective cohort study was conducted between 2022 and 2023 in a single high-volume tertiary center, including 42 patients (54 knees) who consented to be enrolled. Patients underwent a series of 3 injections of ACP at weekly intervals, prepared using the Arthrex ACP Double-Syringe System. Lysholm scores were collected at baseline, 3-months, and 6-months post-injection. Results Forty patients (49 knees) completed the follow-up and were included in the final analysis. The mean age was 53.8 ± 10.16 years (range 35-76 years), and the median body mass index (BMI) was 29 (interquartile range [IQR]: 27-34). There were 22 females and 18 males. Treatment failure occurred in 12 out of 49 cases (24.49%). The mean platelet concentration in the ACP was 588.5 ± 183.2 × 10 6 /ml, with a mean platelet fold increase of 2.14 ± 0.71 compared to the baseline. Multi-linear regression modeling showed that older age and higher mean platelet concentration were predictors of higher post-injection Lysholm scores, with beta coefficients of 0.34 and 0.28, respectively, and p values of 0.013 and 0.036, respectively. Conclusion Autologous conditioned plasma provided clinical benefits in this cohort study of knee OA patients for at least 6 months post-injection. Older age and a higher mean platelet concentration in the ACP were identified as predictors of a higher Lysholm score.

Citation

Selim, A., Lan, T., Hulme, C., Williams, M., Perry, J., Gallacher, P., Jermin, P., & Wright, K. (2025). Clinical and Cellular Predictors of Outcomes in Autologous Conditioned Plasma Therapy for Knee Osteoarthritis: A Prospective Cohort Study. Cartilage, https://doi.org/10.1177/19476035251323376

• Journal Article Type: Article
• Acceptance Date: Mar 19, 2025
• Online Publication Date: Mar 19, 2025
• Publication Date: Mar 1, 2025
• Deposit Date: Apr 1, 2025
• Publicly Available Date: Apr 1, 2025
• Journal: CARTILAGE
• Print ISSN: 1947-6035
• Electronic ISSN: 1947-6043
• Publisher: SAGE Publications
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1177/19476035251323376
• Keywords: autologus conditioned plasma, osteoarthritis, diagnosis, knee, joint involved, clinical research knee, clinical research, clinical research cell based therapies
• Public URL: https://keele-repository.worktribe.com/output/1112424
• Publisher URL: https://journals.sagepub.com/doi/10.1177/19476035251323376