CRISPR/Cas9-Mediated Knockout of the Corazonin Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (Schistocerca gregaria)

He, Yingying; Yan, Qiang; Bi, Yong; Liu, Guosheng; Hou, Shuang; Chen, Xinyi; Zhao, Xiaoming; Zhang, Xueyao; Zhang, Min; Zhang, Jianzhen; Ma, Binbin; Warren, Benjamin; Roth, Siegfried; Zhang, Tingting

Abstract

The desert locust (Schistocerca gregaria) represents one of the most destructive agricultural pests globally, renowned for its ability to form massive swarms that can devastate crops and threaten food security across vast regions. Despite the widespread application of the CRISPR/Cas9 gene-editing system in several insect orders, its utilization in locusts, particularly in the desert locust, has remained relatively unexplored. We established a CRISPR/Cas9-mediated gene-editing workflow for the desert locust using gene encoding for neuropeptide corazonin (Crz) as a target. We also analyzed the phenotypic and physiological characteristics of the mutant using paraffin sectioning, HE staining, and chitin staining techniques. Our findings revealed that while Crz knockout desert locusts were viable and maintained normal fertility, they exhibited striking phenotypic alterations, including albinism and a significant reduction in cuticle thickness. These observations not only highlight the functional role of Crz in pigmentation and cuticle development but also underscore the potential of CRISPR/Cas9 as a powerful tool for dissecting gene function in locusts. Furthermore, the successful application of CRISPR/Cas9 in desert locusts also paves the way for similar genetic studies in other non-model insects, expanding the scope of functional genomics in entomology.

Citation

He, Y., Yan, Q., Bi, Y., Liu, G., Hou, S., Chen, X., Zhao, X., Zhang, X., Zhang, M., Zhang, J., Ma, B., Warren, B., Roth, S., & Zhang, T. (2025). CRISPR/Cas9-Mediated Knockout of the Corazonin Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (Schistocerca gregaria). Insects, 16(7), 1-14. https://doi.org/10.3390/insects16070704

• Journal Article Type: Article
• Acceptance Date: Jul 7, 2025
• Online Publication Date: Jul 9, 2025
• Publication Date: 2025
• Deposit Date: Jul 31, 2025
• Journal: Insects
• Electronic ISSN: 2075-4450
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 7
• Article Number: 704
• Pages: 1-14
• DOI: https://doi.org/10.3390/insects16070704
• Keywords: CRISPR/Cas9, corazonin, Schistocerca gregaria, cuticle
• Public URL: https://keele-repository.worktribe.com/output/1328308