Skip to main content

Research Repository

Advanced Search

Small-Molecule-Induced Clustering of Heparan Sulfate Promotes Cell Adhesion

Takemoto, Naohiro; Suehara, Tetsuya; Frisco, Heidie L.; Sato, Shin-ichi; Sezaki, Takuhito; Kusamori, Kosuke; Kawazoe, Yoshinori; Min Park, Sun; Yamazoe, Sayumi; Mizuhata, Yoshiyuki; Inoue, Rintaro; Miller, Gavin J.; Hansen, Steen U.; Jayson∥, Gordon C.; Gardiner, John M.; Kanaya, Toshiji; Tokitoh, Norihiro; Ueda, Kazumitsu; Takakura, Yoshinobu; Kioka, Noriyuki; Nishikawa, Makiya; Uesugi, Motonari

Authors

Naohiro Takemoto

Tetsuya Suehara

Heidie L. Frisco

Shin-ichi Sato

Takuhito Sezaki

Kosuke Kusamori

Yoshinori Kawazoe

Sun Min Park

Sayumi Yamazoe

Yoshiyuki Mizuhata

Rintaro Inoue

Steen U. Hansen

Gordon C. Jayson∥

John M. Gardiner

Toshiji Kanaya

Norihiro Tokitoh

Kazumitsu Ueda

Yoshinobu Takakura

Noriyuki Kioka

Makiya Nishikawa

Motonari Uesugi



Abstract

Adhesamine is an organic small molecule that promotes adhesion and growth of cultured human cells by binding selectively to heparan sulfate on the cell surface. The present study combined chemical, physicochemical, and cell biological experiments, using adhesamine and its analogues, to examine the mechanism by which this dumbbell-shaped, non-peptidic molecule induces physiologically relevant cell adhesion. The results suggest that multiple adhesamine molecules cooperatively bind to heparan sulfate and induce its assembly, promoting clustering of heparan sulfate-bound syndecan-4 on the cell surface. A pilot study showed that adhesamine improved the viability and attachment of transplanted cells in mice. Further studies of adhesamine and other small molecules could lead to the design of assembly-inducing molecules for use in cell biology and cell therapy.

Citation

Takemoto, N., Suehara, T., Frisco, H. L., Sato, S., Sezaki, T., Kusamori, K., …Uesugi, M. (2013). Small-Molecule-Induced Clustering of Heparan Sulfate Promotes Cell Adhesion. Journal of the American Chemical Society, 135(30), 11032 -11039. https://doi.org/10.1021/ja4018682

Journal Article Type Article
Acceptance Date Jul 3, 2013
Online Publication Date Jul 17, 2013
Publication Date Jul 31, 2013
Journal Journal Of The American Chemical Society
Print ISSN 0002-7863
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 135
Issue 30
Pages 11032 -11039
DOI https://doi.org/10.1021/ja4018682
Publisher URL http://dx.doi.org/10.1021/ja4018682