The Metabolic Fate and Effects of 2-Bromophenol in Male Sprague-Dawley Rats.

Thompson, David F.

Abstract

1. The metabolic fate and urinary excretion of 2-bromophenol, a phenolic metabolite of bromobenzene, was investigated in male Sprague Dawley rats following single intraperitoneal doses at either 0, 100 or 200?mg/kg. 2. Urine was collected for seven days and samples analysed using 1H NMR spectroscopy, inductively coupled plasma (ICP)MS, and UPLC-MS. 3. 1H NMR spectroscopy of the urine samples showed that, at these doses, 2-bromophenol had little effect on endogenous metabolite profiles, supporting histopathology and clinical chemistry data which showed no changes associated with the administration of 2-bromophenol at these doses. 4. The use of ICP-MS Provided a means for the selective detection and quantification of bromine-containing species and showed that between 15 and 30% of the dose was excreted via the urine over the 7 days of the study for both the 100 and 200?mg doses respectively. 6. The bulk of the excretion of Br-containing material had occurred by 8?hr post administration. UPLC-MS of urine revealed a number of metabolites of 2-bromophenol, with 2-bromophenol glucuronide and 2-bromophenol sulphate identified as the major species. A number of minor hydroxylated metabolites were also detected as their glucuronide, sulphate or O-methyl conjugates. There was no evidence for the production of reactive metabolites.

Citation

Thompson, D. F. (2019). The Metabolic Fate and Effects of 2-Bromophenol in Male Sprague-Dawley Rats. Xenobiotica, 1352-1359. https://doi.org/10.1080/00498254.2018.1559376

• Acceptance Date: Dec 12, 2018
• Publication Date: Nov 2, 2019
• Journal: Xenobiotica
• Print ISSN: 0049-8254
• Publisher: Taylor and Francis
• Pages: 1352-1359
• DOI: https://doi.org/10.1080/00498254.2018.1559376
• Keywords: 2-Bromobenzene, metabolism, nephrotoxicity, metabonomics
• Publisher URL: https://doi.org/10.1080/00498254.2018.1559376