Antifouling performance and mechanism analysis of marine peptide modified aluminum alloy surface

Lou, Tong; Bai, Xiuqin; He, Xiaoyan; Liu, Wencheng; Yang, Ying; Yuan, Chengqing

Abstract

Biofouling, the settlement of nuisance fouling organisms on underwater surfaces, seriously affects the performance of the ship and the development of the maritime industry. Using antimicrobial peptides to obtain antifouling performance is one of the most effective methods to solve this challenge. The present work assessed the peptide with the key amino acid residues and sequence (LWFYTMWH) of the marine microalgae Tetraselmis suecica as the antifoulants to aluminum alloy. The aluminum surface was introduced Alsingle bondC bond through reduction of aryldiazonium salts. The peptides were grafted onto the surfaces by covalently binding the amino groups of peptides with the carboxyl groups on surfaces via 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) activation. The ATR-FTIR and XPS analyses confirmed the successful modification of peptides on the surfaces. The as-modified surfaces showed good antifouling capacity against the settlement of Gram-positive bacteria (Bacillus sp.) and Gram-negative bacteria (Escherichia coli) by destroying the cell membranes. The antibacterial mechanism of peptides was explained by coarse-grained molecular dynamics simulations and atomistic molecular dynamics simulations. It was revealed that the peptides mainly interacted with the phospholipid membranes through the amino residues at the carboxyl terminus, resulting in changes in the thickness and local curvature of the phospholipid membranes, eventually causing the membranes to rupture.

• Journal Article Type: Article
• Acceptance Date: Jul 21, 2022
• Publication Date: 2022-09
• Deposit Date: Jun 1, 2023
• Journal: Surface and Coatings Technology
• Print ISSN: 0257-8972
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 445
• Pages: 128742
• DOI: https://doi.org/10.1016/j.surfcoat.2022.128742
• Keywords: Materials Chemistry; Surfaces, Coatings and Films; Surfaces and Interfaces; Condensed Matter Physics; General Chemistry
• Additional Information: This article is maintained by: Elsevier; Article Title: Antifouling performance and mechanism analysis of marine peptide modified aluminum alloy surface; Journal Title: Surface and Coatings Technology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.surfcoat.2022.128742; Content Type: article; Copyright: © 2022 Published by Elsevier B.V.