Development and Application of an NMR-Based Assay for Polyphenol Oxidases

Li, Yu; Zafar, Ayesha; Kilmartin, Paul A.; Reynisson, Jóhannes; Leung, Ivanhoe K. H.

Abstract

Polyphenol oxidases (PPOs) are enzymes that catalyse the oxidation of phenolic compounds. We report a NMR-based assay that can be used as a screening and validation tool for PPO activity modulators, which was demonstrated using a series of PPO inhibitors and antioxidants. This method is exemplified by work in tandem with virtual screening and molecular modelling, which led to the discovery of several novel PPO inhibitors.

Citation

Li, Y., Zafar, A., Kilmartin, P. A., Reynisson, J., & Leung, I. K. H. (2017). Development and Application of an NMR-Based Assay for Polyphenol Oxidases. ChemistrySelect, 2(32), 10435-10441. https://doi.org/10.1002/slct.201702144

• Journal Article Type: Article
• Online Publication Date: Nov 13, 2017
• Publication Date: Nov 13, 2017
• Deposit Date: Jun 8, 2023
• Journal: ChemistrySelect
• Print ISSN: 2365-6549
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 32
• Pages: 10435-10441
• DOI: https://doi.org/10.1002/slct.201702144
• Keywords: General Chemistry