Fabrication and characterization of 3D complex hydroxyapatite scaffolds with hierarchical porosity of different features for optimal bioactive performance

Chen, Zhichao; Zhang, Xianglin; Yang, Yang; Zhou, Kui; Wragg, Nicholas; Liu, Yang; Lewis, Mark; Liu, Changqing

Abstract

To improve the biological performance of hydroxyapatite scaffolds in bone tissue engineering, graphite was used as porogen to create additional micro/nanoporosity to macroporosity, resulting in hierarchical porosity. For maximum imitation of natural bone structures, scaffolds with different porosity features were fabricated using micron/nano-sized graphite. The sintering profile of graphite treated scaffolds was optimised to reduce the influence of shrinkage. To confirm the porosity features, the micro/nanostructures of scaffolds were characterised by scanning electron microscopy and Brunauer-Emmett-Teller method. Considering that hydroxyapatite is resistant to biodegradation in vivo, the degradation rate of scaffolds in modified simulated body fluid was examined. Furthermore, biological evaluations based on myoblasts were carried out to investigate the influence of porosity features on the essential performance such as adhesion, proliferation and differentiation. The results indicate that the scaffolds with dominant microporosity and little nanoporosity formed inside had high potential for clinical applications due to improved performance in bioactivity.

Citation

Chen, Z., Zhang, X., Yang, Y., Zhou, K., Wragg, N., Liu, Y., Lewis, M., & Liu, C. (2017). Fabrication and characterization of 3D complex hydroxyapatite scaffolds with hierarchical porosity of different features for optimal bioactive performance. Ceramics International, 43(1 Part A), 336-344. https://doi.org/10.1016/j.ceramint.2016.09.160

• Journal Article Type: Article
• Publication Date: 2017-01
• Deposit Date: Jun 9, 2023
• Journal: Ceramics International
• Print ISSN: 0272-8842
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 1 Part A
• Pages: 336-344
• DOI: https://doi.org/10.1016/j.ceramint.2016.09.160
• Keywords: Materials Chemistry; Surfaces, Coatings and Films; Process Chemistry and Technology; Ceramics and Composites; Electronic, Optical and Magnetic Materials; Hydroxyapatite scaffold; Hierarchical porosity; Porosity feature; Biological performance; Bone tissue engineering
• Public URL: https://keele-repository.worktribe.com/output/444312
• Additional Information: This article is maintained by: Elsevier; Article Title: Fabrication and characterization of 3D complex hydroxyapatite scaffolds with hierarchical porosity of different features for optimal bioactive performance; Journal Title: Ceramics International; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.ceramint.2016.09.160; Content Type: article; Copyright: © 2016 Elsevier Ltd and Techna Group S.r.l. All rights reserved.