Precision control of dissolved oxygen in mammalian cell culture media impacts on in situ volatile generation and promotes improved mesenchymal stem cell yield accompanied by reduced transcriptional variability

Mohammad, Al Zubaidi; Sule Suso, Josep; Forsyth, Nicholas

Abstract

Mammalian cell culture is reliant on culture media to support in vitro growth and proliferation. Culture media contains salts, vitamins, and minerals at precise levels where subtle variations result in novel media formulations. Oxygen is a bioactive molecule which impacts strongly on intracellular signalling, mitochondrial function, and metabolic pathway choices. We sought to determine the levels of dissolved oxygen in cell culture media and to design systems to support precision control of those levels.

Citation

Mohammad, A. Z., Sule Suso, J., & Forsyth, N. (2015). Precision control of dissolved oxygen in mammalian cell culture media impacts on in situ volatile generation and promotes improved mesenchymal stem cell yield accompanied by reduced transcriptional variability. Free Radical Biology and Medicine, 86(S1), S16. https://doi.org/10.1016/j.freeradbiomed.2015.07.069

• Journal Article Type: Conference Paper
• Conference Name: SFRR-E/SNFS Conference
• Conference Location: Stuttgart, Germany
• Online Publication Date: Aug 28, 2015
• Publication Date: 2015-09
• Deposit Date: Jun 12, 2023
• Journal: Free Radical Biology and Medicine
• Print ISSN: 0891-5849
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 86
• Issue: S1
• Pages: S16
• DOI: https://doi.org/10.1016/j.freeradbiomed.2015.07.069
• Keywords: Physiology (medical); Biochemistry