Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen

Chapman, Michael R.; Cosgrove, Sebastian C.; Turner, Nicholas J.; Kapur, Nikil; Blacker, A. John

Abstract

We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O2 directly in reaction media, thereby enabling the concentration of aqueous O2 to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L−1 h−1). This scalable benchtop method provides a distinct advantage over conventional bio-oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space-time yields for bio-oxidation.

Citation

Chapman, M. R., Cosgrove, S. C., Turner, N. J., Kapur, N., & Blacker, A. J. (2018). Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen. Angewandte Chemie International Edition, 57(33), 10535-10539. https://doi.org/10.1002/anie.201803675

• Journal Article Type: Article
• Acceptance Date: May 9, 2018
• Online Publication Date: May 9, 2018
• Publication Date: Aug 13, 2018
• Deposit Date: Jun 15, 2023
• Journal: Angewandte Chemie International Edition
• Print ISSN: 1433-7851
• Electronic ISSN: 1521-3773
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 33
• Pages: 10535-10539
• DOI: https://doi.org/10.1002/anie.201803675
• Keywords: General Chemistry; Catalysis
• Additional Information: Received: 2018-03-27; Published: 2018-06-14