Initial observations of dynamically heated bone: Initial observations of dynamically heated bone

Greenwood, C.; Rogers, K.; Beckett, S.; Clement, J.

Abstract

Sufferers of cystic fibrosis are at extremely high risk for contracting chronic lung infections. Over their lifetime, one bacterial strain in particular, Pseudomonas aeruginosa, becomes the dominant pathogen. Bacterial strains incur loss-of-function mutations in the mucA gene that lead to a mucoid conversion, resulting in copious secretion of the exopolysaccharide alginate. Strategies that stop the production of alginate in mucoid Pseudomonas aeruginosa infections are therefore of paramount importance. To aid in this, a series of sugar nucleotide tools to probe an enzyme critical to alginate biosynthesis, guanosine diphosphate mannose dehydrogenase (GMD), have been developed. GMD catalyzes the irreversible formation of the alginate building block, guanosine diphosphate mannuronic acid. Using a chemoenzymatic strategy, we accessed a series of modified sugar nucleotides, identifying a C6-amide derivative of guanosine diphosphate mannose as a micromolar inhibitor of GMD. This discovery provides a framework for wider inhibition strategies against GMD to be developed.

• Journal Article Type: Article
• Acceptance Date: Nov 19, 2020
• Online Publication Date: Nov 12, 2013
• Publication Date: 2013-12
• Deposit Date: Jun 16, 2023
• Journal: Crystal Research and Technology
• Print ISSN: 0232-1300
• Publisher: Wiley-VCH Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 48
• Issue: 12
• Pages: 1073-1082
• DOI: https://doi.org/10.1002/crat.201300254
• Keywords: Condensed Matter Physics; General Materials Science; General Chemistry