Physical stability, biocompatibility and potential use of hybrid iron oxide-gold nanoparticles as drug carriers

Barnett, Christopher M.; Gueorguieva, Mariana; Lees, Martin R.; McGarvey, David J.; Hoskins, Clare

Abstract

Hybrid nanoparticles (HNPs) such as iron oxide-gold nanoparticles are currently being exploited for their potential application in image-guided therapies. However, little investigation has been carried out into their physical or chemical stability and potential cytotoxicity in biological systems. Here, we determine the HNPs physical stability over 6 months and chemical stability in physiological conditions, and estimate the biological activity of uncoated and poly(ethylene glycol) coated nanoparticles on human pancreatic adenocarcinoma (BxPC-3) and differentiated human monocyte cells (U937). The potential of these HNPs to act as drug carrier vehicles was determined using the model drug 6-Thioguanine (6-TG). The data showed that the HNPs maintained their structural integrity both physically and chemically throughout the duration of the studies. In addition, negligible cytotoxicity or free radical production was observed in the cell lines tested. The 6-TG was successfully conjugated; with a ratio of 3:1:10 Fe:Au:6-TG (wt:wt:wt). After incubation with BxPC-3 cells, enhanced cellular uptake was reported with the 6-TG-conjugated HNPs compared with free drug along with a 10-fold decrease in IC50. This exciting data highlights the potential of HNPs for use in image-guided drug delivery.

• Journal Article Type: Article
• Acceptance Date: May 6, 2013
• Online Publication Date: May 15, 2013
• Publication Date: 2013-06
• Deposit Date: Jun 16, 2023
• Journal: Journal of Nanoparticle Research
• Print ISSN: 1388-0764
• Electronic ISSN: 1572-896X
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 6
• DOI: https://doi.org/10.1007/s11051-013-1706-2
• Keywords: Condensed Matter Physics; General Materials Science; Modeling and Simulation; General Chemistry; Atomic and Molecular Physics, and Optics; Bioengineering