Inhibition of the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa Using Targeted Sugar Nucleotide Probes

Beswick, Laura; Dimitriou, Eleni; Ahmadipour, Sanaz; Zafar, Ayesha; Rejzek, Martin; Reynisson, Johannes; Field, Robert; Miller, Gavin

Abstract

Sufferers of cystic fibrosis are at extremely high risk for contracting chronic lung infections. Over their lifetime, one bacterial strain in particular, Pseudomonas aeruginosa, becomes the dominant pathogen. Bacterial strains incur loss-of-function mutations in the mucA gene that lead to a mucoid conversion, resulting in copious secretion of the exopolysaccharide alginate. Strategies that stop the production of alginate in mucoid Pseudomonas aeruginosa infections are therefore of paramount importance. To aid in this, a series of sugar nucleotide tools to probe an enzyme critical to alginate biosynthesis, guanosine diphosphate mannose dehydrogenase (GMD), have been developed. GMD catalyzes the irreversible formation of the alginate building block, guanosine diphosphate mannuronic acid. Using a chemoenzymatic strategy, we accessed a series of modified sugar nucleotides, identifying a C6-amide derivative of guanosine diphosphate mannose as a micromolar inhibitor of GMD. This discovery provides a framework for wider inhibition strategies against GMD to be developed.

Citation

Beswick, L., Dimitriou, E., Ahmadipour, S., Zafar, A., Rejzek, M., Reynisson, J., …Miller, G. (2020). Inhibition of the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa Using Targeted Sugar Nucleotide Probes. ACS chemical biology, 15(12), 3086–3092. https://doi.org/10.1021/acschembio.0c00426

• Journal Article Type: Article
• Acceptance Date: Nov 19, 2020
• Online Publication Date: Nov 25, 2020
• Publication Date: Dec 18, 2020
• Deposit Date: Jun 16, 2023
• Journal: ACS Chemical Biology
• Print ISSN: 1554-8929
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 12
• Pages: 3086–3092
• DOI: https://doi.org/10.1021/acschembio.0c00426
• Keywords: sugar nucleotides, Glycosyl Phosphates Mannosyl, Chemical Tools, bacterial biosynthesis, enzyme inhibition, Carbohydrate Synthesis
• Publisher URL: https://pubs.acs.org/doi/10.1021/acschembio.0c00426
• Related Public URLs: https://chemrxiv.org/engage/chemrxiv/article-details/60c74baebb8c1ab8d23db274
• Additional Information: ChemRxiv is a preprint server.; Preprint DOI; 10.26434/chemrxiv.12369758.v1