Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes

Mourtada-Maarabouni, Mirna; Keen, Jennifer; Clark, Jeremy; Cooper, Colin S.; Williams, Gwyn T.

Abstract

RBM5 (RNA-binding motif protein 5/LUCA-15/H37) is encoded at the lung cancer tumor suppressor locus 3p21.3 and itself has several important characteristics of a tumor suppressor, including both potentiation of apoptosis and inhibition of the cell cycle. Here, we report the effects of both upregulation and downregulation of LUCA-15/RBM5 on gene expression monitored using cDNA microarrays. Many of the genes modulated by LUCA-15/RBM5 are involved in the control of apoptosis, the cell cycle, or both. These effects were confirmed for the most significant genes using real-time RT-PCR and/or Western blotting. In particular, LUCA-15/RBM5 increased the expression of Stat5b and BMP5 and decreased the expression of AIB1 (Amplified In Breast Cancer 1), proto-oncogene Pim-1, caspase antagonist BIRC3 (cIAP-2, MIHC), and CDK2 (cyclin-dependent kinase 2). These effects on multiple genes controlling both apoptosis and proliferation are in line with the functional effects of LUCA-15/RBM5 and indicate that it plays a central role in regulating cell fate consistent with its tumor suppressor activity.

Citation

Mourtada-Maarabouni, M., Keen, J., Clark, J., Cooper, C. S., & Williams, G. T. (2006). Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes. Experimental Cell Research, 312(10), 1745-1752. https://doi.org/10.1016/j.yexcr.2006.02.009

• Journal Article Type: Article
• Acceptance Date: Feb 8, 2006
• Online Publication Date: Mar 20, 2006
• Publication Date: Jun 10, 2006
• Deposit Date: May 16, 2024
• Journal: Experimental Cell Research
• Print ISSN: 0014-4827
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 312
• Issue: 10
• Pages: 1745-1752
• ISBN: 00144827
• DOI: https://doi.org/10.1016/j.yexcr.2006.02.009
• Public URL: https://keele-repository.worktribe.com/output/543764
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0014482706000498