Isolation of genes controlling apoptosis through their effects on cell survival

Williams, Gwyn T.; Hughes, Jane P.; Stoneman, Victoria; Anderson, Claire L.; McCarthy, Nicola J.; Mourtada-Maarabouni, Mirna; Pickard, Mark; Hedge, Vanessa L.; Trayner, Ian; Farzaneh, Farzin

Abstract

The identification of the most suitable molecular targets for gene and drug therapy is the crucial first step in the development of new disease treatments. The rational identification of such targets depends on a detailed understanding of the pathological changes occuring at the molecular level. We have applied forward genetics approaches to the identification of the critical genes involved in the control of apoptosis in mammalian cells, since defective control of apoptosis underlies many diseases, including cancer and neurodegenerative diseases. We have identified two groups of genes by their effects on cell survival using retroviral cDNA functional expression cloning and retroviral insertional mutagenesis. The identification of these novel genes opens up new areas for apoptosis research and subsequently for the development of new gene and drug therapies.

Citation

Williams, G. T., Hughes, J. P., Stoneman, V., Anderson, C. L., McCarthy, N. J., Mourtada-Maarabouni, M., …Farzaneh, F. (2006). Isolation of genes controlling apoptosis through their effects on cell survival. Gene Therapy and Molecular Biology, 10(28), 255-262

• Journal Article Type: Article
• Publication Date: 2006
• Deposit Date: May 16, 2024
• Journal: Gene Therapy and Molecular Biology
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 28
• Pages: 255-262
• ISBN: 15299120
• Keywords: apoptosis, forward genetics, functional cloning, retroviral insertional mutagenesis, oncogenes, tumour suppressor genes
• Public URL: https://keele-repository.worktribe.com/output/543770
• Publisher URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828370/
• PMID: 17372619