On 2.5D surface reconstruction of cell cultures

Smith, W. A.; Lam, K. P.; Collins, D. J.; Richardson, J. B.

Abstract

The domain of image processing for cell microscopy presents non-trivial challenges that must be addressed for consistent image quality. One such challenge concerns the loss of focus as a result of cellular processes, where cell objects may move or change their morphology and, as a result, lie outside of the depth-of-view of the lens. This paper presents two approaches to addressing this problem; namely, the multiscale and geometric methods of image depth estimation. These algorithms are applied to a z-stack of images acquired from a standard phase contrast microscope and a total internal reflection microscope. To assess the algorithms in terms of their scalability, 10×, 20×, and 60× lens objectives are used to offer increased spatial resolutions as well as corresponding improvements in image quality.

Citation

Smith, W. A., Lam, K. P., Collins, D. J., & Richardson, J. B. (2013). On 2.5D surface reconstruction of cell cultures. In 2013 8th International Symposium on Image and Signal Processing and Analysis (ISPA). https://doi.org/10.1109/ispa.2013.6703742

• Conference Name: 2013 8th International Symposium on Image and Signal Processing and Analysis (ISPA)
• Conference Location: Trieste, Italy
• Start Date: Sep 4, 2013
• End Date: Sep 6, 2013
• Online Publication Date: Jan 9, 2014
• Publication Date: 2013-09
• Deposit Date: Dec 15, 2023
• Publisher: Institute of Electrical and Electronics Engineers (IEEE)
• Book Title: 2013 8th International Symposium on Image and Signal Processing and Analysis (ISPA)
• DOI: https://doi.org/10.1109/ispa.2013.6703742
• Publisher URL: https://ieeexplore.ieee.org/document/6703742