Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment.

Čermáková, Eliška; Mukherjee, Subham; Nováková, Denisa; Horká, Petra; Zdeňková, Kamila; Demnerová, Kateřina

Abstract

Fish from the pike ( ) genus are valued in gastronomy for their superior meat quality. However, they can cause allergic reactions in sensitive consumers. This work aimed to fill the gap in the detection of pike allergens using molecular-biological techniques. New, fast, and accurate loop-mediated isothermal amplification (LAMP) and real-time PCR (qPCR) assays were designed to detect pike DNA using the parvalbumin gene as a marker. LAMP was assessed by electrophoresis, SYBR green optical detection, and real-time fluorescence detection. The latter was the most sensitive, detecting as little as 0.78 ng of pike DNA; the qPCR detection limit was 0.1 ng. The LAMP analysis took 20-70 min, which is significantly faster than qPCR. The study provides reliable detection and quantification of the parvalbumin gene in both fresh and processed samples and further highlights the versatility of the use of the parvalbumin gene for the authentication of food products and consumer protection via refined allergen risk assessment that is independent of the type of tissue or food processing method used.

Citation

Čermáková, E., Mukherjee, S., Nováková, D., Horká, P., Zdeňková, K., & Demnerová, K. (2024). Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment. Journal of Agricultural and Food Chemistry, 72(22), 12788–12797. https://doi.org/10.1021/acs.jafc.4c01410

• Journal Article Type: Article
• Acceptance Date: May 14, 2024
• Online Publication Date: May 23, 2024
• Publication Date: Jun 5, 2024
• Deposit Date: Jun 10, 2024
• Journal: Journal of agricultural and food chemistry
• Print ISSN: 0021-8561
• Electronic ISSN: 1520-5118
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 72
• Issue: 22
• Pages: 12788–12797
• DOI: https://doi.org/10.1021/acs.jafc.4c01410
• Keywords: LAMP, DNA, food fraud, Esox, food allergy, PCR
• Public URL: https://keele-repository.worktribe.com/output/847400
• Publisher URL: https://pubs.acs.org/doi/10.1021/acs.jafc.4c01410