Skip to main content

Research Repository

Advanced Search

Osteoinductive potential of graphene and graphene oxide for bone tissue engineering: a comparative study

Kashte, Shivaji Bhikaji; Kadam, Sachin; Maffulli, Nicola; Potty, Anish G.; Migliorini, Filippo; Gupta, Ashim

Authors

Shivaji Bhikaji Kashte

Sachin Kadam

Nicola Maffulli

Anish G. Potty

Filippo Migliorini

Ashim Gupta



Abstract

Background
Bone defects, especially critical-size bone defects, and their repair pose a treatment challenge. Osteoinductive scaffolds have gained importance given their potential in bone tissue engineering applications.

Methods
Polycaprolactone (PCL) scaffolds are used for their morphological, physical, cell-compatible and osteoinductive properties. The PCL scaffolds were prepared by electrospinning, and the surface was modified by layer-by-layer deposition using either graphene or graphene oxide.

Results
Graphene oxide-coated PCL (PCL-GO) scaffolds showed a trend for enhanced physical properties such as fibre diameter, wettability and mechanical properties, yield strength, and tensile strength, compared to graphene-modified PCL scaffolds (PCL-GP). However, the surface roughness of PCL-GP scaffolds showed a higher trend than PCL-GO scaffolds. In vitro studies showed that both scaffolds were cell-compatible. Graphene oxide on PCL scaffold showed a trend for enhanced osteogenic differentiation of human umbilical cord Wharton’s jelly-derived Mesenchymal Stem Cells without any differentiation media than graphene on PCL scaffolds after 21 days.

Conclusion
Graphene oxide showed a trend for higher mineralisation, but this trend is not statistically significant. Therefore, graphene and graphene oxide have the potential for bone regeneration and tissue engineering applications. Future in vivo studies and clinical trials are warranted to justify their ultimate clinical use.

Citation

Kashte, S. B., Kadam, S., Maffulli, N., Potty, A. G., Migliorini, F., & Gupta, A. (2024). Osteoinductive potential of graphene and graphene oxide for bone tissue engineering: a comparative study. Journal of Orthopaedic Surgery and Research, 19(1), 1-11. https://doi.org/10.1186/s13018-024-05028-9

Journal Article Type Article
Acceptance Date Aug 22, 2024
Online Publication Date Aug 30, 2024
Publication Date Aug 30, 2024
Deposit Date Sep 11, 2024
Publicly Available Date Sep 11, 2024
Journal Journal of Orthopaedic Surgery and Research
Electronic ISSN 1749-799X
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 19
Issue 1
Article Number 527
Pages 1-11
DOI https://doi.org/10.1186/s13018-024-05028-9
Keywords Osteoinductivity, Graphene, Umbilical cord, Osteogenic differentiation, Scaffolds, Bone defects, Bone tissue engineering
Public URL https://keele-repository.worktribe.com/output/892006
Publisher URL https://josr-online.biomedcentral.com/articles/10.1186/s13018-024-05028-9

Files

Osteoinductive potential of graphene and graphene oxide for bone tissue engineering: a comparative study (1.5 Mb)
Archive

Licence
https://creativecommons.org/licenses/by/4.0/

Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.






You might also like



Downloadable Citations