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Developing a Biomimetic 3D Neointimal Layer as a Prothrombotic Substrate for a Humanized In Vitro Model of Atherothrombosis (2024)
Journal Article
Echrish, J., Pasca, M.-I., Cabrera, D., Yang, Y., & Harper, A. G. S. (2024). Developing a Biomimetic 3D Neointimal Layer as a Prothrombotic Substrate for a Humanized In Vitro Model of Atherothrombosis. Biomimetics, 9(6), Article 372. https://doi.org/10.3390/biomimetics9060372

Acute cardiovascular events result from clots caused by the rupture and erosion of atherosclerotic plaques. This paper aimed to produce a functional biomimetic hydrogel of the neointimal layer of the atherosclerotic plaque that can support thrombogen... Read More about Developing a Biomimetic 3D Neointimal Layer as a Prothrombotic Substrate for a Humanized In Vitro Model of Atherothrombosis.

Magnetic Coagulometry: Towards a New Nanotechnological Tool for ex vivo Monitoring Coagulation in Human Whole Blood (2023)
Journal Article
Santana-Otero, A., Harper, A., Telling, N. D., Ortega, D., & Cabrera, D. (in press). Magnetic Coagulometry: Towards a New Nanotechnological Tool for ex vivo Monitoring Coagulation in Human Whole Blood. Nanoscale, https://doi.org/10.1039/d3nr02593d

Blood clotting disorders consisting of unwanted blood clot formation or excessive bleeding are some of the main causes of death worldwide. However, there are significant limitations in the current methods used to clinically monitoring the dynamics of... Read More about Magnetic Coagulometry: Towards a New Nanotechnological Tool for ex vivo Monitoring Coagulation in Human Whole Blood.

Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models (2023)
Journal Article
Ranjbar, J., Njoroge, W., Gibbins, J. M., Roach, P., Yang, Y., & Harper, A. G. S. (2023). Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models. Gels, 9(6), Article 477. https://doi.org/10.3390/gels9060477

Current in vitro thrombosis models utilise simplistic 2D surfaces coated with purified components of the subendothelial matrix. The lack of a realistic humanised model has led to greater study of thrombus formation in in vivo tests in animals. Here w... Read More about Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models.

Clot-Targeted Magnetic Hyperthermia Permeabilizes Blood Clots to Make Them More Susceptible to Thrombolysis (2022)
Journal Article
Cabrera, D., Sharifabad, M. E., Ranjbar, J. A., Telling, N. D., & Harper, A. G. (2022). Clot-Targeted Magnetic Hyperthermia Permeabilizes Blood Clots to Make Them More Susceptible to Thrombolysis. Journal of Thrombosis and Haemostasis, 20(11), https://doi.org/10.1111/jth.15846

BACKGROUND: Thrombolysis is a frontline treatment for stroke, which involves the application of tissue plasminogen activator (tPA) to trigger endogenous clot-degradation pathways.However, it is only effective within 4.5 hours of symptom onset due to... Read More about Clot-Targeted Magnetic Hyperthermia Permeabilizes Blood Clots to Make Them More Susceptible to Thrombolysis.

The Combination of Tissue-Engineered Blood Vessel Constructs and Parallel Flow Chamber Provides a Potential Alternative to In Vivo Drug Testing Models (2021)
Journal Article
Njoroge, W., Hernández Hernández, A. C., Idris Musa, F., Butler, R., Harper, A. G. S., & Yang, Y. (2021). The Combination of Tissue-Engineered Blood Vessel Constructs and Parallel Flow Chamber Provides a Potential Alternative to In Vivo Drug Testing Models. Pharmaceutics, 13(3), Article 340. https://doi.org/10.3390/pharmaceutics13030340

Cardiovascular disease is a major cause of death globally. This has led to significant efforts to develop new anti-thrombotic therapies or re-purpose existing drugs to treat cardiovascular diseases. Due to difficulties of obtaining healthy human bloo... Read More about The Combination of Tissue-Engineered Blood Vessel Constructs and Parallel Flow Chamber Provides a Potential Alternative to In Vivo Drug Testing Models.

Controlling human platelet activation with calcium-binding nanoparticles (2020)
Journal Article
Cabrera, D., Walker, K., Moise, S., Telling, N. D., & Harper, A. G. (2020). Controlling human platelet activation with calcium-binding nanoparticles. Nano Research, 13, Article 2697–2705. https://doi.org/10.1007/s12274-020-2912-8

We introduce a neural method that is able to fuse concepts from a knowledge base with the context information for the task of grouping of aspect terms. Rather than only using context information, we use the corresponding concepts of aspect terms as a... Read More about Controlling human platelet activation with calcium-binding nanoparticles.

Human platelets use a cytosolic Ca2+ nanodomain to activate Ca2+-dependent shape change independently of platelet aggregation (2020)
Journal Article
Harper. (2020). Human platelets use a cytosolic Ca2+ nanodomain to activate Ca2+-dependent shape change independently of platelet aggregation. Cell Calcium, 90, Article 102248. https://doi.org/10.1016/j.ceca.2020.102248

Human platelets use a rise in cytosolic Ca2+ concentration to activate all stages of thrombus formation, however, how they are able to decode cytosolic Ca2+ signals to trigger each of these independently is unknown. Other cells create local Ca2+ sign... Read More about Human platelets use a cytosolic Ca2+ nanodomain to activate Ca2+-dependent shape change independently of platelet aggregation.

A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall. (2016)
Journal Article
Yang, Y., Musa, F., & Harper, A. (2016). A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall. Tissue Engineering Part C: Methods, 691 - 699. https://doi.org/10.1089/ten.tec.2015.0582

Native blood vessels contain both an antiaggregatory intimal layer, which prevents platelet activation in the intact vessel, and a proaggregatory medial layer, which stimulates platelet aggregation upon vascular damage. Yet, current techniques for as... Read More about A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall..

Islet-intrinsic effects of CFTR mutation (2016)
Journal Article
Harper. (2016). Islet-intrinsic effects of CFTR mutation. Diabetologia, 1350-1355. https://doi.org/10.1007/s00125-016-3936-1

Cystic fibrosis-related diabetes (CFRD) is the most significant extra-pulmonary comorbidity in cystic fibrosis (CF) patients, and accelerates lung decline. In addition to the traditional view that CFRD is a consequence of fibrotic destruction of the... Read More about Islet-intrinsic effects of CFTR mutation.

Nicergoline inhibits human platelet Ca2+ signalling through triggering a microtubule-dependent reorganisation of the platelet ultrastructure (2015)
Journal Article
Harper. (2015). Nicergoline inhibits human platelet Ca2+ signalling through triggering a microtubule-dependent reorganisation of the platelet ultrastructure. British Journal of Pharmacology, 234-247. https://doi.org/10.1111/bph.13361

BACKGROUND AND PURPOSE: Recently, we demonstrated that a pericellular Ca(2+) recycling system potentiates agonist-evoked Ca(2+) signalling and granule secretion in human platelets and hypothesized a role for the membrane complex (MC) in orchestratin... Read More about Nicergoline inhibits human platelet Ca2+ signalling through triggering a microtubule-dependent reorganisation of the platelet ultrastructure.

Conventional Protein Kinase C isoforms differentially regulate ADP- and thrombin-evoked Ca2+ signalling in human platelets (2015)
Journal Article
Harper. (2015). Conventional Protein Kinase C isoforms differentially regulate ADP- and thrombin-evoked Ca2+ signalling in human platelets. Cell Calcium, 577-588. https://doi.org/10.1016/j.ceca.2015.09.005

Rises in cytosolic Ca2+ concentration ([Ca2+]cyt) are central in platelet activation, yet many aspects of the underlying mechanisms are poorly understood. Most studies examine how experimental manipulations affect agonist-evoked rises in [Ca2+]cyt, b... Read More about Conventional Protein Kinase C isoforms differentially regulate ADP- and thrombin-evoked Ca2+ signalling in human platelets.

Pericellular Ca(2+) recycling potentiates thrombin-evoked Ca(2+) signals in human platelets. (2013)
Journal Article
Sage, S., Pugh, N., Farndale, R., & Harper, A. (2013). Pericellular Ca(2+) recycling potentiates thrombin-evoked Ca(2+) signals in human platelets. https://doi.org/10.1002/phy2.85

We have previously demonstrated that Na(+)/Ca(2+) exchangers (NCXs) potentiate Ca(2+) signaling evoked by thapsigargin in human platelets, via their ability to modulate the secretion of autocoids from dense granules. This link was confirmed in platel... Read More about Pericellular Ca(2+) recycling potentiates thrombin-evoked Ca(2+) signals in human platelets..