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All Outputs (24)

Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury (2020)
Journal Article
Prager, J., Adams, C., Delaney, A., Chanoit, G., Tarlton, J., Wong, L., …Granger, N. (2020). Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury. Journal of Tissue Engineering, 11, https://doi.org/10.1177/2041731420934806

Safe hydrogel delivery requires stiffness-matching with host tissues to avoid iatrogenic damage and reduce inflammatory reactions. Hydrogel-encapsulated cell delivery is a promising combinatorial approach to spinal cord injury therapy, but a lack of... Read More about Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury.

Less is more: Investigating the influence of cellular nanoparticle load on transfection outcomes in neural cells (2019)
Journal Article
Chari, D. M., & Tickle, J. A. (2019). Less is more: Investigating the influence of cellular nanoparticle load on transfection outcomes in neural cells. Journal of Tissue Engineering and Regenerative Medicine, https://doi.org/10.1002/term.2909

Genetic engineering of cell transplant populations offers potential for delivery of neurotherapeutic factors to modify the regenerative microenvironment of the injured spinal cord. The use of magnetic nanoparticle (MNP) based vectors has reduced the... Read More about Less is more: Investigating the influence of cellular nanoparticle load on transfection outcomes in neural cells.

Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices (2019)
Journal Article
Adams, C., Delaney, A., Carwardine, D., Tickle, J., Granger, N., & Chari, D. (2019). Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices. Macromolecular Bioscience, 19, e1800389. https://doi.org/10.1002/mabi.201800389

A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due t... Read More about Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices.

A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells. (2018)
Journal Article
Al-Mayyahi, R. S., Sterio, L. D., Connolly, J. B., Adams, C., Al-Tumah, W. A., Sen, J., …Chari, D. (2018). A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells. Molecular and Cellular Neuroscience, 86, 30-40. https://doi.org/10.1016/j.mcn.2017.11.006

Corticosteroids (CSs) are widely used clinically, for example in pediatric respiratory distress syndrome, and immunosuppression to prevent rejection of stem cell transplant populations in neural cell therapy. However, such treatment can be associated... Read More about A proteomic investigation into mechanisms underpinning corticosteroid effects on neural stem cells..

Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles (2017)
Journal Article
Evans, M., Shakli, A., Jenkins, S., & Chari, D. (2017). Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles. Nano Research, 2881-2890. https://doi.org/10.1007/s12274-017-1496-4

The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction m... Read More about Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.

Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes. (2017)
Book Chapter
Pickard, M., Adams, C., & Chari, D. (2017). Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes. In Current Protocols in Stem Cell Biology (2D.19.1 - 2D.19.16). https://doi.org/10.1002/9780470151808

Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs)... Read More about Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes..

Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology. (2016)
Journal Article
Adams, C., Dickson, A., Kuiper, J., & Chari, D. (2016). Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology. Nanoscale, 8, 17869-17880. https://doi.org/10.1039/c6nr05244d

Tissue engineering studies are witnessing a major paradigm shift to cell culture on biomimetic materials that replicate native tissue features from which the cells are derived. Few studies have been performed in this regard for neural cells, particul... Read More about Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology..

Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology (2016)
Journal Article
Fernandes, A. R., & Chari, D. M. (2016). Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology. Journal of Controlled Release, 300-310. https://doi.org/10.1016/j.jconrel.2016.06.039

Both neurotrophin-based therapy and neural stem cell (NSC)-based strategies have progressed to clinical trials for treatment of neurological diseases and injuries. Brain-derived neurotrophic factor (BDNF) in particular can confer neuroprotective and... Read More about Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology.

Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy (2016)
Journal Article
Chari, & Fernandes, A. R. (2016). Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy. Journal of Controlled Release, 289-299. https://doi.org/10.1016/j.jconrel.2016.06.024

Genetically engineered neural stem cell (NSC) transplant populations offer key benefits in regenerative neurology, for release of therapeutic biomolecules in ex vivo gene therapy. NSCs are ‘hard-to-transfect’ but amenable to ‘magnetofection’. Despite... Read More about Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy.

Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake (2016)
Journal Article
Weightman, A. P., Jenkins, S. I., & Chari, D. M. (2016). Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake. Nano Research, 9(8), 2384-2397. https://doi.org/10.1007/s12274-016-1125-7

Development of nanoparticle (NP) based therapies to promote regeneration in sites of central nervous system (CNS; i.e. brain and spinal cord) pathology relies critically on the availability of experimental models that offer biologically valid predict... Read More about Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake.

Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. (2016)
Journal Article
Tickle, J., Jenkins, S., Polyak, B., Pickard, M., & Chari, D. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 345 - 358. https://doi.org/10.2217/nnm.15.202

AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of var... Read More about Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance..

'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics (2016)
Journal Article
Jenkins, S., Weinberg, D., Al-Shakli, A., Fernandes, A., Yiu, H., Telling, N., …Chari, D. (2016). 'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics. Journal of Controlled Release, 224, 136-145. https://doi.org/10.1016/j.jconrel.2016.01.013

Surface engineering to control cell behavior is of high interest across the chemical engineering, drug delivery and biomaterial communities. Defined chemical strategies are necessary to tailor nanoscale protein interactions/adsorption, enabling contr... Read More about 'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics.

Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures (2015)
Journal Article
Weinberg, D., Adams, C., & Chari, D. (2015). Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures. RSC advances, 5, 43353-43360. https://doi.org/10.1039/c5ra07481a

Neural stem cells (NSCs) have a high therapeutic potential for patients with neurological disease/injury given their neuroregenerative and immunomodulatory capabilities. In recent years, magnetic nanoparticles (MNPs) have been used as contrast agents... Read More about Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures.

Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes (2015)
Journal Article
Pickard, M., Adams, C., Barraud, P., & Chari, D. (2015). Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes. Journal of Functional Biomaterials, 6, 259-279. https://doi.org/10.3390/jfb6020259

Genetically engineered neural stem cell (NSC) transplants offer a key strategy to augment neural repair by releasing therapeutic biomolecules into injury sites. Genetic modification of NSCs is heavily reliant on viral vectors but cytotoxic effects ha... Read More about Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes.

Development of a nanomaterial bio-screening platform for neurological applications (2015)
Journal Article
Jenkins, S., Roach, P., & Chari, D. (2015). Development of a nanomaterial bio-screening platform for neurological applications. Nanomedicine: Nanotechnology, Biology and Medicine, 77 - 87. https://doi.org/10.1016/j.nano.2014.07.010

Nanoparticle platforms are being intensively investigated for neurological applications. Current biological models used to identify clinically relevant materials have major limitations, e.g. technical/ethical issues with live animal experimentation,... Read More about Development of a nanomaterial bio-screening platform for neurological applications.

Magnetic nanoparticles for oligodendrocyte precursor cell transplantation therapies: progress and challenges (2014)
Journal Article
Jenkins, S., Yiu, H., Rosseinsky, M., & Chari, D. (2014). Magnetic nanoparticles for oligodendrocyte precursor cell transplantation therapies: progress and challenges. Molecular and Cellular Therapies, https://doi.org/10.1186/2052-8426-2-23

Oligodendrocyte precursor cells (OPCs) have shown high promise as a transplant population to promote regeneration in the central nervous system, specifically, for the production of myelin - the protective sheath around nerve fibers. While clinical tr... Read More about Magnetic nanoparticles for oligodendrocyte precursor cell transplantation therapies: progress and challenges.