Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans

Desbois, Muriel; Pak, Joseph S.; Opperman, Karla J.; Giles, Andrew C.; Grill, Brock

doi:10.1016/j.xpro.2023.102262

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Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans (2023)
Journal Article
Desbois, M., Pak, J. S., Opperman, K. J., Giles, A. C., & Grill, B. (2023). Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans. STAR Protocols, 4(2), https://doi.org/10.1016/j.xpro.2023.102262

We present an optimized protocol for in vivo affinity purification proteomics and biochemistry using the model organism C. elegans. We describe steps for target tagging, large-scale culture, affinity purification using a cryomill, mass spectrometry a... Read More about Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans.