Outputs (8)

Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency (2022)
Journal Article
Barreto, S., Al-Zubaidi, M., Dale, T., Worrall, A., Kapacee, Z., Kimber, S., …Rutter, A. (2022). Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency. Preprints, https://doi.org/10.20944/preprints202203.0143.v1

Human pluripotent stem cells (hPSCs) have widespread potential biomedical applications. There is a need for large-scale in vitro production of hPSCs, and optimal culture methods are vital in achieving this. Physiological oxygen (2% O2) improves key h... Read More about Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency.

Optimisation of sample preparation using glass slides for spectral pathology (2020)
Journal Article
Dowling, L., Roach, P., Rutter, A., Yousef, I., Pillai, S., Latham, D., …Sule-Suso, J. (2020). Optimisation of sample preparation using glass slides for spectral pathology. Applied Spectroscopy, 75(3), 343-350. https://doi.org/10.1177/0003702820945748

The clinical translation of Fourier Transform Infrared (FTIR) microspectroscopy in pathology will require bringing this technique as close as possible to standard practice in pathology departments. An important step is sample preparation for both FTI... Read More about Optimisation of sample preparation using glass slides for spectral pathology.

Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model. (2018)
Journal Article
Owida, H., Rutter, A., Cinque, G., Kuiper, N., Sule-Suso, J., & Yang, Y. (2018). Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model. Analyst, 5979-5986. https://doi.org/10.1039/c8an01272e

Isolated and monolayer expanded chondrocytes are not the ideal cell form to produce a cartilage matrix. In articular cartilage, each chondrocyte is surrounded by a 2-4 µm thick collagen VI-rich pericellular matrix (PCM) forming a chondron. Freshly ex... Read More about Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model..

Evaluation of peroxidative stress of cancer cells in vitro by real-time quantification of volatile aldehydes in culture headspace (2017)
Journal Article
Shestivska, V., Rutter, A. V., Sule-Suso, J., Smith, D., & Spanel, P. (2017). Evaluation of peroxidative stress of cancer cells in vitro by real-time quantification of volatile aldehydes in culture headspace. Rapid Communications in Mass Spectrometry, 31(16), 1344-1352. https://doi.org/10.1002/rcm.7911

Rationale Peroxidation of lipids in cellular membranes results in the release of volatile organic compounds (VOCs), including saturated aldehydes. The real-time quantification of trace VOCs produced by cancer cells during peroxidative stress present... Read More about Evaluation of peroxidative stress of cancer cells in vitro by real-time quantification of volatile aldehydes in culture headspace.

Effects of nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning (2016)
Journal Article
Siddique, M., Rutter, A., Wehbe, K., Cinque, G., Bellisola, G., & Sule-Suso, J. (2016). Effects of nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning. Analyst, 8(142), 1299-1307. https://doi.org/10.1039/c6an01914e

Over the last few years, both synchrotron-based FTIR (S-FTIR) and Raman microspectroscopies have helped to better understand the effects of drugs on cancer cells. However, cancer is a mixture of cells with different sensitivity/resistance to drugs. F... Read More about Effects of nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning.

Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy (2014)
Journal Article
Rutter, A. V., Siddique, M. R., Filik, J., Sandt, C., Dumas, P., Cinque, G., …Sulé-Suso, J. (2014). Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy. Cytometry Part A, 85(8), 688-697. https://doi.org/10.1002/cyto.a.22488

Over the last few years, significant scientific insight on the effects of chemotherapy drugs at cellular level using synchrotron-based FTIR (S-FTIR) microspectroscopy has been obtained. The work carried out so far has identified spectral differences... Read More about Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy.

Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation (2013)
Journal Article
Pijanka, J. K., Stone, N., Rutter, A. V., Forsyth, N., Sockalingum, G. D., Yang, Y., & Sulé-Suso, J. (2013). Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation. Analyst, 138(17), 5052. https://doi.org/10.1039/c3an00968h

Raman spectroscopy has been widely used to study its possible clinical application in cancer diagnosis. However, in order to make it into clinical practice, it is important that this technique is able not only to identify cancer cells from their norm... Read More about Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation.

Quantification by SIFT-MS of acetaldehyde released by lung cells in a 3D model (2012)
Journal Article
Rutter, A. V., Chippendale, T. W. E., Yang, Y., Španěl, P., Smith, D., & Sulé-Suso, J. (in press). Quantification by SIFT-MS of acetaldehyde released by lung cells in a 3D model. Analyst, 138(1), 91-95. https://doi.org/10.1039/c2an36185j

Our previous studies have shown that both lung cancer cells and non-malignant lung cells release acetaldehyde in vitro. However, data from other laboratories have produced conflicting results. Furthermore, all these studies have been carried out in 2... Read More about Quantification by SIFT-MS of acetaldehyde released by lung cells in a 3D model.