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Human mesenchymal stem cells stimulate EaHy926 endothelial cell migration: combined proteomic and in vitro analysis of the influence of donor-donor variability

Walter, MNM; Kohli, N; Major, T; Fuller, H; Wright, KT; Kuiper, J; Johnson, WEB

Human mesenchymal stem cells stimulate EaHy926 endothelial cell migration: combined proteomic and in vitro analysis of the influence of donor-donor variability Thumbnail


Authors

MNM Walter

N Kohli

T Major

WEB Johnson



Abstract

Mesenchymal stem cells (MSCs) stimulate angiogenesis within a wound environment and this effect is mediated through paracrine interactions with the endothelial cells present. Here we report that human MSC-conditioned medium (n=3 donors) significantly increased EaHy-926 endothelial cell adhesion and cell migration, but that this stimulatory effect was markedly donor-dependent. MALDI-TOF/TOF mass spectrometry demonstrated that whilst collagen type I and fibronectin were secreted by all of the MSC cultures, the small leucine rich proteoglycan, decorin was secreted only by the MSC culture that was least effective upon EaHy-926 cells. These individual extracellular matrix components were then tested as culture substrata. EaHy-926 cell adherence was greatest on fibronectin-coated surfaces with least adherence on decorin-coated surfaces. Scratch wound assays were used to examine cell migration. EaHy-926 cell scratch wound closure was quickest on substrates of fibronectin and slowest on decorin. However, EaHy-926 cell migration was stimulated by the addition of MSC-conditioned medium irrespective of the types of culture substrates. These data suggest that whilst the MSC secretome may generally be considered angiogenic, the composition of the secretome is variable and this variation probably contributes to donor-donor differences in activity. Hence, screening and optimizing MSC secretomes will improve the clinical effectiveness of pro-angiogenic MSC-based therapies.

Citation

Walter, M., Kohli, N., Major, T., Fuller, H., Wright, K., Kuiper, J., & Johnson, W. (2015). Human mesenchymal stem cells stimulate EaHy926 endothelial cell migration: combined proteomic and in vitro analysis of the influence of donor-donor variability. Journal of Stem Cells and Regenerative Medicine, 11(1), 18-24. https://doi.org/10.46582/jsrm.1101004

Journal Article Type Article
Acceptance Date Jan 24, 2015
Online Publication Date May 30, 2015
Publication Date May 30, 2015
Journal Journal of Stem Cells and Regenerative Medicine
Print ISSN 0973-7154
Publisher German Society for Stem Cell Research (GSZ)
Peer Reviewed Peer Reviewed
Volume 11
Issue 1
Pages 18-24
DOI https://doi.org/10.46582/jsrm.1101004
Keywords mesenchymal stem cell, EaHy-926 Endothelial cell, angiogenesis, secretome, donor variability
Publisher URL http://www.pubstemcell.com/monthly/011010300004.htm

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