DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS

Glossop, John R; Haworth, Kim E; Emes, Richard D; Nixon, Nicola B; Packham, Jon C; Dawes, Peter T; Fryer, Anthony A; Mattey, Derek L; Farrell, William E

Abstract

AIM: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). We profiled genome-wide DNA methylation in these cells from synovial fluid, a more readily accessible source of disease-associated cells. PATIENTS & METHODS: Genome-wide DNA methylation was interrogated in fluid-derived FLS from five RA and six osteoarthritis patients using Human Methylation 450 Bead Chip and bisulfite pyrosequencing. RESULTS: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and osteoarthritis fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (˜40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes. CONCLUSION: Synovial fluid-derived RA FLS show methylation changes common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.

Citation

Glossop, J. R., Haworth, K. E., Emes, R. D., Nixon, N. B., Packham, J. C., Dawes, P. T., …Farrell, W. E. (2015). DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7(4), 539 -551. https://doi.org/10.2217/epi.15.15

• Journal Article Type: Article
• Online Publication Date: Jun 25, 2015
• Publication Date: Jun 1, 2015
• Journal: Epigenomics
• Print ISSN: 1750-1911
• Publisher: Future Medicine
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 4
• Pages: 539 -551
• DOI: https://doi.org/10.2217/epi.15.15
• Keywords: DNA methylation, epigenetics, fibroblast-like synoviocyte, osteoarthritis, rheumatoid arthritis, synovial fluid
• Publisher URL: http://dx.doi.org/10.2217/epi.15.15
• PMID: 26111028