Engineering CRISPR guide RNA riboswitches for in vivo applications

Galizi, Roberto

Abstract

CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implications for both basic and applied research, spanning from fundamental genetic studies and synthetic biology to genetic editing and gene therapy.

Citation

Galizi, R., & Jaramillo, A. (2018). Engineering CRISPR guide RNA riboswitches for in vivo applications. Current Opinion in Biotechnology, 103 - 113. https://doi.org/10.1016/j.copbio.2018.08.007

• Journal Article Type: Article
• Acceptance Date: Aug 16, 2018
• Publication Date: Sep 25, 2018
• Publicly Available Date: Jun 28, 2023
• Journal: Current Opinion in Biotechnology
• Print ISSN: 0958-1669
• Publisher: Elsevier
• Pages: 103 - 113
• DOI: https://doi.org/10.1016/j.copbio.2018.08.007
• Keywords: CRISPR, RNA, Riboswitches.
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0958166918300569?via%3Dihub