3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells.

Camilla Ciardulli, Maria; Lovecchio, Joseph; Scala, Pasqualina; Pavel Lamparelli, Erwin; Dale, Tina; Giudice, Valentina; Giordano, Emanuele; Selleri, Carmine; Robert Forsyth, Nicholas; Maffulli, Nicola; Della Porta, Giovanna

Abstract

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton's Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p = 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p = 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-ß1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

Citation

Camilla Ciardulli, M., Lovecchio, J., Scala, P., Pavel Lamparelli, E., Dale, T., Giudice, V., …Della Porta, G. (2021). 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells. Pharmaceutics, 13(9), https://doi.org/10.3390/pharmaceutics13091448

• Journal Article Type: Article
• Acceptance Date: Sep 8, 2021
• Publication Date: Sep 10, 2021
• Journal: Pharmaceutics
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 9
• DOI: https://doi.org/10.3390/pharmaceutics13091448
• Keywords: human Wharton's Jelly Mesenchymal Stem Cells; hGDF-5 controlled delivery; PLGA nanocarriers; 3D fibrin scaffold; tenogenic commitment; cyclic strain bioreactor systems
• Publisher URL: https://doi.org/10.3390/pharmaceutics13091448