Maria Camilla Ciardulli
3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells.
Camilla Ciardulli, Maria; Lovecchio, Joseph; Scala, Pasqualina; Pavel Lamparelli, Erwin; Dale, Tina; Giudice, Valentina; Giordano, Emanuele; Selleri, Carmine; Robert Forsyth, Nicholas; Maffulli, Nicola; Della Porta, Giovanna
Authors
Joseph Lovecchio
Pasqualina Scala
Erwin Pavel Lamparelli
Tina Dale t.p.dale@keele.ac.uk
Valentina Giudice
Emanuele Giordano
Carmine Selleri
Nicholas Robert Forsyth
Nicola Maffulli
Giovanna Della Porta
Abstract
The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton's Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p = 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p = 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-ß1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.
Citation
Camilla Ciardulli, M., Lovecchio, J., Scala, P., Pavel Lamparelli, E., Dale, T., Giudice, V., …Della Porta, G. (2021). 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells. Pharmaceutics, 13(9), https://doi.org/10.3390/pharmaceutics13091448
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 8, 2021 |
Publication Date | Sep 10, 2021 |
Journal | Pharmaceutics |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Issue | 9 |
DOI | https://doi.org/10.3390/pharmaceutics13091448 |
Keywords | human Wharton's Jelly Mesenchymal Stem Cells; hGDF-5 controlled delivery; PLGA nanocarriers; 3D fibrin scaffold; tenogenic commitment; cyclic strain bioreactor systems |
Publisher URL | https://doi.org/10.3390/pharmaceutics13091448 |
Files
pharmaceutics-13-01448-v2.pdf
(68.8 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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