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3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells.

Camilla Ciardulli, Maria; Lovecchio, Joseph; Scala, Pasqualina; Pavel Lamparelli, Erwin; Dale, Tina; Giudice, Valentina; Giordano, Emanuele; Selleri, Carmine; Robert Forsyth, Nicholas; Maffulli, Nicola; Della Porta, Giovanna

3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells. Thumbnail


Authors

Maria Camilla Ciardulli

Joseph Lovecchio

Pasqualina Scala

Erwin Pavel Lamparelli

Valentina Giudice

Emanuele Giordano

Carmine Selleri

Nicholas Robert Forsyth

Nicola Maffulli

Giovanna Della Porta



Abstract

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton's Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p = 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p = 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-ß1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

Journal Article Type Article
Acceptance Date Sep 8, 2021
Publication Date Sep 10, 2021
Journal Pharmaceutics
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 13
Issue 9
DOI https://doi.org/10.3390/pharmaceutics13091448
Keywords human Wharton's Jelly Mesenchymal Stem Cells; hGDF-5 controlled delivery; PLGA nanocarriers; 3D fibrin scaffold; tenogenic commitment; cyclic strain bioreactor systems
Publisher URL https://doi.org/10.3390/pharmaceutics13091448

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