A Tailor-Made Synthetic Polymer for Cell Encapsulation: Design Rationale, Synthesis, Chemical-Physics and Biological Characterizations

Gerges, Irini; Tamplenizza, Margherita; Rossi, Eleonora; Tocchio, Alessandro; Martello, Federico; Recordati, Camilla; Kumar, Deepak; Forsyth, Nicholas R.; Liu, Yang; Lenardi, Cristina

Abstract

This study presents a custom-made in situ gelling polymeric precursor for cell encapsulation. Composed of poly((2-hydroxyethyl)methacrylate-co-(3-aminopropyl)methacrylamide) (P(HEMA-co-APM) mother backbone and RGD-mimicking poly(amidoamine) (PAA) moiteis, the comb-like structured polymeric precursor is tailored to gather the advantages of the two families of synthetic polymers, i.e., the good mechanical integrity of PHEMA-based polymers and the biocompatibility and biodegradability of PAAs. The role of P(HEMA-co-APM) in the regulation of the chemico-physical properties of P(HEMA-co-APM)/PAA hydrogels is thoroughly investigated. On the basis of obtained results, namely the capability of maintaining vital NIH3T3 cell line in vitro for 2 d in a 3D cell culture, the in vivo biocompatibility in murine model for 16 d, and the ability of finely tuning mechanical properties and degradation kinetics, it can be assessed that P(HEMA-co-APM)/PAAs offer a cost-effective valid alternative to the so far studied natural polymer-based systems for cell encapsulation.

Citation

Gerges, I., Tamplenizza, M., Rossi, E., Tocchio, A., Martello, F., Recordati, C., …Lenardi, C. (2016). A Tailor-Made Synthetic Polymer for Cell Encapsulation: Design Rationale, Synthesis, Chemical-Physics and Biological Characterizations. Macromolecular Bioscience, 16(6), 870-881. https://doi.org/10.1002/mabi.201500386

• Journal Article Type: Article
• Online Publication Date: Feb 22, 2016
• Publication Date: 2016-06
• Deposit Date: Jun 7, 2023
• Journal: Macromolecular Bioscience
• Print ISSN: 1616-5187
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 6
• Pages: 870-881
• DOI: https://doi.org/10.1002/mabi.201500386
• Keywords: Materials Chemistry; Polymers and Plastics; Biomaterials; Bioengineering; Biotechnology