Amphiregulin co-operates with bone morphogenetic protein 15 to increase bovine oocyte developmental competence: effects on gap junction-mediated metabolite supply

Sugimura, Satoshi; Ritter, Lesley J.; Sutton-McDowall, Melanie L.; Mottershead, David G.; Thompson, Jeremy G.; Gilchrist, Robert B.

Abstract

This study assessed the participation of amphiregulin (AREG) and bone morphogenetic protein 15 (BMP15) during maturation of bovine cumulus-oocyte complexes (COCs) on cumulus cell function and their impact on subsequent embryo development. AREG treatment of COCs enhanced blastocyst formation and quality only when in the presence of BMP15. Expression of hyaluronan synthase 2 was enhanced by follicle-stimulating hormone (FSH) but not by AREG, which was reflected in the level of cumulus expansion. Although both FSH and AREG stimulated glycolysis, AREG-treated COCs had higher glucose consumption, lactate production and ratio of lactate production to glucose uptake. Autofluorescence levels in oocytes, indicative of NAD(P)H and FAD++, were increased with combined AREG and BMP15 treatment of COCs. In contrast, these treatments did not alter autofluorescence levels when cumulus cells were removed from oocytes, even in the presence of other COCs, suggesting that oocyte-cumulus gap-junctional communication (GJC) is required. FSH contributed to maintaining GJC for an extended period of time. Remarkably, BMP15 was equally effective at maintaining GJC even in the presence of AREG. Hence, AREG stimulation of COC glycolysis and BMP15 preservation of GJC may facilitate efficient transfer of metabolites from cumulus cells to the oocyte thereby enhancing oocyte developmental competence. These results have implications for improving in vitro oocyte maturation systems.

Citation

Sugimura, S., Ritter, L. J., Sutton-McDowall, M. L., Mottershead, D. G., Thompson, J. G., & Gilchrist, R. B. (2014). Amphiregulin co-operates with bone morphogenetic protein 15 to increase bovine oocyte developmental competence: effects on gap junction-mediated metabolite supply. Molecular Human Reproduction, 20(6), 499-513. https://doi.org/10.1093/molehr/gau013

• Journal Article Type: Article
• Online Publication Date: Feb 19, 2014
• Publication Date: 2014-06
• Deposit Date: Jun 7, 2023
• Journal: MHR: Basic science of reproductive medicine
• Print ISSN: 1360-9947
• Electronic ISSN: 1360-9947
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Issue: 6
• Pages: 499-513
• DOI: https://doi.org/10.1093/molehr/gau013
• Keywords: Cell Biology; Developmental Biology; Obstetrics and Gynecology; Genetics; Molecular Biology; Embryology; Reproductive Medicine; amphiregulin; BMP15; cumulus-oocyte complex; gap-junction; metabolism