Dendrimer Conjugate of [4-(Tetradecanoylamino)benzyl]phosphonic Acid (S32826) as an Autotaxin Inhibitor.

Fisher, Natalie; Hilton-Bolt, Timothy; G Edwards, Michael; J Haxton, Katherine; McKenzie, Michael; Allin, Steven M.; Richardson, Alan

Abstract

Autotaxin is an extracellular phospholipase D that catalyzes the hydrolysis of lysophosphatidyl choline (LPC) to bioactive lipid lysophosphatidic acid (LPA). LPA has been implicated in many pathological processes relevant to cancer, including cell migration and invasion, proliferation, and survival. The most potent autotaxin inhibitor described to date is the LPA analogue S32826 (IC50 5.6 nM). S32826 and many other autotaxin inhibitors are notably lipophilic, creating a need to improve their physical properties. Polymers are becoming an increasingly useful tool in the delivery of drugs and have the potential to improve the properties of small molecules. Herein we report the synthesis of a S32826 dendrimer conjugate and its biological evaluation. The conjugate was found to inhibit autotaxin activity using two different substrates and to decrease the migration of an ovarian cancer cell line modified to overexpress autotaxin. Furthermore, the conjugate potentiated activation of caspase 3/7 induced by carboplatin.

Citation

Fisher, N., Hilton-Bolt, T., G Edwards, M., J Haxton, K., McKenzie, M., Allin, S. M., & Richardson, A. (2014). Dendrimer Conjugate of [4-(Tetradecanoylamino)benzyl]phosphonic Acid (S32826) as an Autotaxin Inhibitor. ACS medicinal chemistry letters, 5(1), 34-39. https://doi.org/10.1021/ml4003106

• Journal Article Type: Article
• Acceptance Date: Nov 18, 2013
• Online Publication Date: Nov 21, 2013
• Publication Date: Jan 9, 2014
• Deposit Date: Jun 8, 2023
• Journal: ACS medicinal chemistry letters
• Print ISSN: 1948-5875
• Electronic ISSN: 1948-5875
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 1
• Pages: 34-39
• DOI: https://doi.org/10.1021/ml4003106
• Keywords: Organic Chemistry; Drug Discovery; Biochemistry
• PMID: 24900771