Fluorescent Monitoring of Poly(lactic-co-glycolic Acid) Degradation and Extracellular Matrix Turnover of Engineered Tissue Constructs

Bardsley, K.; El Haj, A.J.; Yang, Y

Abstract

Development of fluorescent tagging techniques for inert polymers is an advantageous tool for monitoring biomaterial degradation online. Here we present a protocol for the fluorescent tagging of poly(lactic-co-glycolic acid) (PLGA) which allows for accurate monitoring of degradation. This was applied to PLGA with various lactic/glycolic acid ratios to determine cellular responses to degradation. The final aim was to produce a new metric, the turnover index (TI), which assesses cellular responses to biomaterial degradation.

Citation

Bardsley, K., El Haj, A., & Yang, Y. (2015, September). Fluorescent Monitoring of Poly(lactic-co-glycolic Acid) Degradation and Extracellular Matrix Turnover of Engineered Tissue Constructs. Paper presented at 2015 4th TERMIS World Congress, Boston, Massachusetts, USA

• Presentation Conference Type: Conference Paper (unpublished)
• Conference Name: 2015 4th TERMIS World Congress
• Start Date: Sep 8, 2015
• End Date: Sep 11, 2015
• Deposit Date: Jun 9, 2023
• Public URL: https://keele-repository.worktribe.com/output/444395
• Publisher URL: https://www.liebertpub.com/doi/10.1089/ten.tea.2015.5000.abstracts