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Microcalcification crystallography as a potential marker of DCIS recurrence

Gosling, Sarah B.; Arnold, Emily L.; Davies, Samantha K.; Cross, Hannah; Bouybayoune, Ihssane; Calabrese, Doriana; Nallala, Jayakrupakar; Pinder, Sarah E.; Fu, Liping; Lips, Esther H.; King, Lorraine; Marks, Jeffrey; Hall, Allison; Grimm, Lars J.; Lynch, Thomas; Pinto, Donna; Stobart, Hilary; Shelley Hwang, E.; Wesseling, Jelle; Geraki, Kalotina; Stone, Nicholas; Lyburn, Iain D.; Greenwood, Charlene; Rogers, Keith D.; PRECISION Consortium, Grand Challenge; Thompson, Alastair; Nik-Zainal, Serena; Sawyer, Elinor J.; Davies, Helen; Futreal, Andrew; Navin, Nicholas; Jonkers, Jos; van Rheenen, Jacco; Behbod, Fariba; Schmidt, Marjanka; Wessels, Lodewyk F.A.; Rea, Daniel; Bhattacharjee, Proteeti; Collyar, Deborah; Verschuur, Ellen; van Oirsouw, Marja

Microcalcification crystallography as a potential marker of DCIS recurrence Thumbnail


Emily L. Arnold

Samantha K. Davies

Hannah Cross

Ihssane Bouybayoune

Doriana Calabrese

Jayakrupakar Nallala

Sarah E. Pinder

Liping Fu

Esther H. Lips

Lorraine King

Jeffrey Marks

Allison Hall

Lars J. Grimm

Thomas Lynch

Donna Pinto

Hilary Stobart

E. Shelley Hwang

Jelle Wesseling

Kalotina Geraki

Nicholas Stone

Iain D. Lyburn

Keith D. Rogers

Grand Challenge PRECISION Consortium

Alastair Thompson

Serena Nik-Zainal

Elinor J. Sawyer

Helen Davies

Andrew Futreal

Nicholas Navin

Jos Jonkers

Jacco van Rheenen

Fariba Behbod

Marjanka Schmidt

Lodewyk F.A. Wessels

Daniel Rea

Proteeti Bhattacharjee

Deborah Collyar

Ellen Verschuur

Marja van Oirsouw


Ductal carcinoma in-situ (DCIS) accounts for 20-25% of all new breast cancer diagnoses. DCIS has an uncertain risk of progression to invasive breast cancer and a lack of predictive biomarkers may result in relatively high levels (~ 75%) of overtreatment. To identify unique prognostic biomarkers of invasive progression, crystallographic and chemical features of DCIS microcalcifications have been explored. Samples from patients with at least 5-years of follow up and no known recurrence (174 calcifications in 67 patients) or ipsilateral invasive breast cancer recurrence (179 microcalcifications in 57 patients) were studied. Significant differences were noted between the two groups including whitlockite relative mass, hydroxyapatite and whitlockite crystal maturity and, elementally, sodium to calcium ion ratio. A preliminary predictive model for DCIS to invasive cancer progression was developed from these parameters with an AUC of 0.797. These results provide insights into the differing DCIS tissue microenvironments, and how these impact microcalcification formation. [Abstract copyright: © 2023. The Author(s).]


Gosling, S. B., Arnold, E. L., Davies, S. K., Cross, H., Bouybayoune, I., Calabrese, D., …van Oirsouw, M. (2023). Microcalcification crystallography as a potential marker of DCIS recurrence. Scientific reports, 13(1), Article 9331.

Journal Article Type Article
Acceptance Date Apr 14, 2023
Online Publication Date Jun 8, 2023
Publication Date Jun 8, 2023
Deposit Date Jun 12, 2023
Publicly Available Date Jun 12, 2023
Journal Scientific Reports
Print ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 13
Issue 1
Article Number 9331
Keywords Crystallography, Tumor Microenvironment, Carcinoma, Intraductal, Noninfiltrating - diagnostic imaging - pathology, Female, Carcinoma, Ductal, Breast - pathology, Neoplasm Recurrence, Local - pathology, Humans, Breast Neoplasms - diagnostic imaging - patho
Additional Information Received: 21 September 2022; Accepted: 14 April 2023; First Online: 8 June 2023; : The authors declare no competing interests.


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