Multi-functional PLGA Scaffolds For Vascularization In Bone Tissue Constructs

Yang, Ying; Lyu, L

Abstract

In our previous work, a new technique in which electrospun nanofibers were rolled into multilayered-rod-like 3D scaffolds has been used in rabbit radius regeneration in vivo. This has been highly successful in bone formation. However these scaffolds have no or low capacity to form blood vessels. With the presence of blood vessels, nutrition and oxygen can be delivered to cells in the implanted scaffolds, therefore enhancing the viability of the implants. In this study, we fabricated new multi-functional PLGA scaffolds with porous and nanofibers (by electrospining) components enabling vascularization along bone formation. PLGA porous component was generated by leaching patterned gelatin particles, which was obtained by microfluidic method. To achieve sustained release and better spatial configuration of VEGF from the scaffolds, VEGF has been immobilized to the PLGA scaffolds through specific binding affinity to heparin which was grafted on the surface of the scaffold covalently. MSCs and HUVECs were cultured on scaffolds to investigate the effects of surface topography, chemistry property, and cell types on cells attachment, viability, and the formation of blood vessel and bone. The results showed that VEGF grafted surface enhanced vascularization greatly on the new PLGA scaffolds in the presence of MSCs or HUVECs or co-culture of both cell types, whilst co-culture showed more organized CD31-positive lumens. Amino group on scaffolds surface combined with co-culture method enhanced osteogenesis of MSCs. Thus, the composite PLGA scaffolds grafted with VEGF can enhance both bone and blood vessel formation, and potentially become a candidate scaffold for bone tissue engineering.

Citation

Yang, Y., & Lyu, L. (2015, September). Multi-functional PLGA Scaffolds For Vascularization In Bone Tissue Constructs

• Presentation Conference Type: Speech
• Start Date: Sep 8, 2015
• End Date: Sep 11, 2015
• Deposit Date: Jun 12, 2023
• Publisher URL: https://www.liebertpub.com/doi/10.1089/ten.tea.2015.5000.abstracts