Preparation and evaluation of PLGA nanoparticle-loaded biodegradable light-responsive injectable implants as a promising platform for intravitreal drug delivery

Bisht, Rohit; Jaiswal, Jagdish K.; Oliver, Verity F.; Eurtivong, Chatchakorn; Reynisson, Johannes; Rupenthal, Ilva D.

Abstract

The present study reports on the development of a hybrid system by integrating poly(lactic-co-glycolic)acid nanoparticles (PLGA NPs) into light-responsive in-situ forming injectable implants (ISFIs) for minimally invasive and safe intravitreal peptide delivery. In the first part of the study, peptide-loaded PLGA based nanoparticles (NPs) were developed using the two-step nanoprecipitation technique. Peptide compatibility with PLGA and polyvinyl alcohol was confirmed via IR spectroscopy. Developed NPs had a size of 149.3–235.4 nm, a polydispersity index between 0.24 and 0.46, a zeta potential of −32.4 to −27.0 mV and an entrapment efficiency of 34.3–55.3%. In the second part of the study, successful synthesis of methacrylated alginate (MA) and its subsequent photocrosslinking upon photoirridiation was confirmed by 1H NMR. Photocrosslinked MA exhibited the required clarity and its gelling time, morphology, syringeability, hardness, rate of swelling and degradation were found suitable for prolonging its residence in the posterior segment. The sulforhodamine B assay on human retinal pigment epithelium cells and the zebrafish embryo toxicity test confirmed the biocompatibility of NPs and ISFIs. Finally, NPs were incorporated into the ISFIs for improved particle retention and to further sustain drug release, suggesting the proposed hybrid system as an innovative and efficient ocular drug delivery platform.

Citation

Bisht, R., Jaiswal, J. K., Oliver, V. F., Eurtivong, C., Reynisson, J., & Rupenthal, I. D. (2017). Preparation and evaluation of PLGA nanoparticle-loaded biodegradable light-responsive injectable implants as a promising platform for intravitreal drug delivery. Journal of Drug Delivery Science and Technology, 40, 142-156. https://doi.org/10.1016/j.jddst.2017.06.006

• Journal Article Type: Article
• Acceptance Date: Jun 11, 2017
• Online Publication Date: Jun 13, 2017
• Publication Date: 2017-08
• Deposit Date: Jun 12, 2023
• Journal: Journal of Drug Delivery Science and Technology
• Print ISSN: 1773-2247
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 40
• Pages: 142-156
• DOI: https://doi.org/10.1016/j.jddst.2017.06.006
• Keywords: Pharmaceutical Science; PLGA nanoparticles; Injectable implant; Methacrylated alginate; Ocular drug delivery; Light-responsive system; In-situ forming hydrogel
• Additional Information: This article is maintained by: Elsevier; Article Title: Preparation and evaluation of PLGA nanoparticle-loaded biodegradable light-responsive injectable implants as a promising platform for intravitreal drug delivery; Journal Title: Journal of Drug Delivery Science and Technology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jddst.2017.06.006; Content Type: article; Copyright: © 2017 Elsevier B.V. All rights reserved.