Investigating the use of marine-derived glycosaminoglycans as mimetics for heparan sulphate in fibroblast growth factor signalling

Chen, Simon

Abstract

Fibroblast growth factors (FGFs) are polypeptide growth factors and are potent regulators of cell proliferation, cell differentiation, cell migration and angiogenesis (Ornitz and Itoh, 2001). FGFs can bind with fibroblast growth factor receptors with the aid of heparan sulphate. As mammalian heparin was known to bind with many FGFs and FGFRs. Marine glycosaminoglycans were tested to see if they could bind with FGF and FGFR to gain greater understanding on the binding between glycosaminoglycans with FGF and FGFR. Marine glycosaminoglycans were tested due to their unique and diverse structures and to test for the biological activity involving with FGF signalling, MTT assay were used in the experiment.

Different variants of polysaccharides derived from Salmon, Cod and Hake were tested. It was shown that all the marine polysaccharide variants were able to promote FGF2 signalling in cells that were transfected in FGFR1c. At a high concentration, all the Cod variants were able to promote FGF2/FGFR2c signalling, and some Salmon variants were not able to promote FGF2 signalling whilst some were able to show some cell proliferation. All the Hake variants were not able to promote FGF2/FGFR2c signalling except for Hake F5. All variants of marine polysaccharides derived from Salmon, Cod and Hake were not able to promote FGF1 signalling in cells transfected with FGFR1c or FGFR2c with the exemption of variant Hake F3.

Citation

Chen, S. (2023). Investigating the use of marine-derived glycosaminoglycans as mimetics for heparan sulphate in fibroblast growth factor signalling. (Thesis). Keele University

• Thesis Type: Thesis
• Deposit Date: Oct 12, 2023
• Publicly Available Date: Oct 12, 2023
• Award Date: 2023-10