Professor Anthony Fryer a.a.fryer@keele.ac.uk
Use of site-directed mutagenesis of allele-specific PCR primers to identify the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms at the glutathione S-transferase, GSTM1 locus
Fryer, A A; Zhao, L; Alldersea, J; Pearson, W R; Strange, R C
Authors
L Zhao
J Alldersea
W R Pearson
R C Strange
Abstract
We describe the identification of the GSTM1 null, GSTM1 A, GSTM1 B and GSTM1 A,B polymorphisms at the glutathione S-transferase GSTM1 locus using a single-step PCR method. Target DNA was amplified using primers to intron 6 and exon 7 with site-directed mutagenesis being used to introduce a restriction site in DNA amplified from GSTM1 *A, thereby allowing differentiation of this allele and GSTM1 *B. The accuracy of this approach in identifying the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms was confirmed by comparison with, firstly, an established PCR method that distinguishes GSTM1 *0 homozygotes from individuals with the other GSTM1 genotypes and, secondly, GSTM1 phenotypes determined using chromatofocusing.
Citation
Fryer, A. A., Zhao, L., Alldersea, J., Pearson, W. R., & Strange, R. C. (1993). Use of site-directed mutagenesis of allele-specific PCR primers to identify the GSTM1 A, GSTM1 B, GSTM1 A,B and GSTM1 null polymorphisms at the glutathione S-transferase, GSTM1 locus. Biochemical Journal, 295(1), 313–315
Journal Article Type | Article |
---|---|
Online Publication Date | Oct 1, 1993 |
Publication Date | 1993-10 |
Deposit Date | Feb 8, 2024 |
Journal | Biochemical Journal |
Print ISSN | 0264-6021 |
Publisher | Portland Press |
Peer Reviewed | Peer Reviewed |
Volume | 295 |
Issue | 1 |
Pages | 313–315 |
Publisher URL | https://portlandpress.com/biochemj/article-abstract/295/1/313/30610/Use-of-site-directed-mutagenesis-of-allele?redirectedFrom=fulltext |
PMID | 8216235 |
You might also like
Monitoring drug interventions in people with bipolar disorder
(2023)
Journal Article
Health Inequality and its link to HbA1c Test Recovery in a Developed Health Economy: In a 'Nearly Post COVID-19' World
(2022)
Presentation / Conference
Variability in test interval is linked to glycated haemoglobin (HbA1c) trajectory over time in people with diabetes
(2022)
Presentation / Conference
Downloadable Citations
About Keele Repository
Administrator e-mail: research.openaccess@keele.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search