Multiparametric Modulation of Magnetic Transduction for Biomolecular Sensing in Liquids

Sanz-de Diego, Elena; Aires, Antonio; Palacios Alonso, Pablo; Cabrera, David; Silvestri, Niccolò; Artés-Ibañez, Emilio J.; Vequi-Suplicy, Cinthia C.; Requejo-Isidro, Jose; Delgado-Buscalioni, Rafael; Pellegrino, Teresa; Cortajarena, Aitziber L.; Teran, Francisco José J

Abstract

The recent COVID19 pandemic has remarkably boosted the research on in vitro diagnosis assays to detect biomarkers in biological fluids. Specificity and sensitivity are mandatory for diagnostic kits aiming to reach clinical stages. Whilst the modulation of sensitivity can significantly improve the detection of biomarkers in liquids, this has been scarcely explored. Here, we report on the proof of concept and parametrization of a novel biosensing methodology based on the changes of AC magnetic hysteresis areas observed for magnetic nanoparticles following biomolecular recognition in liquids. Several parameters are shown to significantly modulate the transducing capacity of magnetic nanoparticles to detect analytes dispersed in saline buffer at concentrations of clinical relevance. Magnetic nanoparticles were bio-conjugated with an engineered recognition peptide as a receptor. Analytes are engineered tetratricopeptide binding domains fused to the fluorescent protein whose dimerization state allows mono- or divalent variants. Our results unveil that the number of receptors per particle, analyte valency and concentration, nanoparticle composition and concentration, and field conditions play a key role in the formation of assemblies driven by biomolecular recognition. Consequently, all these parameters modulate the nanoparticle transduction capacity. Our study provides essential insights into the potential of AC magnetometry for customizing biomarker detection in liquids.

Citation

Sanz-de Diego, E., Aires, A., Palacios Alonso, P., Cabrera, D., Silvestri, N., Artés-Ibañez, E. J., …Teran, F. J. J. (2024). Multiparametric Modulation of Magnetic Transduction for Biomolecular Sensing in Liquids. Nanoscale, 16(8), 4082-4094. https://doi.org/10.1039/d3nr06489a

• Journal Article Type: Article
• Acceptance Date: Jan 24, 2024
• Online Publication Date: Jan 25, 2024
• Publication Date: 2024
• Deposit Date: Jan 29, 2024
• Publicly Available Date: Jan 26, 2025
• Journal: Nanoscale
• Print ISSN: 2040-3364
• Electronic ISSN: 2040-3372
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 8
• Pages: 4082-4094
• DOI: https://doi.org/10.1039/d3nr06489a
• Keywords: General Materials Science
• Public URL: https://keele-repository.worktribe.com/output/715238
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2024/nr/d3nr06489a
• Additional Information: Please consider RSC copyright policy: RSC copyright policy: Accepted manuscripts may be distributed via repositories after an embargo period of 12 months