Molecular and Serological Surveillance for Mycobacterium leprae and Mycobacterium lepromatosis in Wild Red Squirrels (Sciurus vulgaris) from Scotland and Northern England

Zhou, Zijie; van Hooij, Anouk; Wassenaar, Gaby N.; Seed, Emma; Verhard-Seymonsbergen, Els M.; Corstjens, Paul L. A. M.; Meredith, Anna L.; Wilson, Liam A.; Milne, Elspeth M.; Beckmann, Katie M.; Geluk, Annemieke

Abstract

Leprosy is a poverty-associated infectious disease in humans caused by Mycobacterium leprae or M. lepromatosis, often resulting in skin and peripheral nerve damage, which remains a significant public health concern in isolated areas of low- and middle-income countries. Previous studies reported leprosy in red squirrels in the British Isles, despite the fact that autochthonous human cases have been absent for centuries in this region. To investigate the extent of M. leprae and M. lepromatosis presence in wild red squirrels in the northern UK, we analyzed 220 blood/body cavity fluid samples from opportunistically sampled red squirrels (2004–2023) for specific antibodies against phenolic glycolipid-I, a cell wall component specific for these leprosy bacilli. Additionally, we assessed bacillus-derived DNA by real-time PCR (qPCR) in 250 pinnae from the same cohort. M. lepromatosis and M. leprae DNA were detected by qPCR in 20.4% and 0.8% of the squirrels, respectively. No cases of co-detection were observed. Detectable levels of anti-PGL-I antibodies by UCP-LFA were observed in 52.9% of animals with the presence of M. lepromatosis determined by qPCR, and overall in 15.5% of all animals. In total, 22.6% (n = 296) of this UK cohort had at least some exposure to leprosy bacilli. Our study shows that leprosy bacilli persist in red squirrels in the northern UK, emphasizing the necessity for ongoing molecular and serological monitoring to study leprosy ecology in red squirrels, gain insight into potential zoonotic transmission, and to determine whether the disease has a conservation impact on this endangered species.

Citation

Zhou, Z., van Hooij, A., Wassenaar, G. N., Seed, E., Verhard-Seymonsbergen, E. M., Corstjens, P. L. A. M., …Geluk, A. (2024). Molecular and Serological Surveillance for Mycobacterium leprae and Mycobacterium lepromatosis in Wild Red Squirrels (Sciurus vulgaris) from Scotland and Northern England. Animals, 14(13), Article 2005. https://doi.org/10.3390/ani14132005

• Journal Article Type: Article
• Acceptance Date: Jul 3, 2024
• Online Publication Date: Jul 7, 2024
• Publication Date: Jul 7, 2024
• Deposit Date: Jul 15, 2024
• Journal: Animals
• Electronic ISSN: 2076-2615
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 13
• Article Number: 2005
• DOI: https://doi.org/10.3390/ani14132005
• Keywords: anti-PGL-I antibody; DNA; leprosy; Mycobacterium leprae; Mycobacterium lepromatosis; red squirrel; real-time PCR; UCP-LFA
• Public URL: https://keele-repository.worktribe.com/output/875604
• Publisher URL: https://www.mdpi.com/2076-2615/14/13/2005