Nanoscale synchrotron x-ray analysis of intranuclear iron in melanised neurons of Parkinson’s substantia nigra

Brooks, Jake; Everett, James; Hill, Emily; Billimoria, Kharmen; Morris, Christopher M.; Sadler, Peter J.; Telling, Neil; Collingwood, Joanna F.

Abstract

Neuromelanin-pigmented neurons of the substantia nigra are selectively lost during the progression of Parkinson’s disease. These neurons accumulate iron in the disease state, and iron-mediated neuron damage is implicated in cell death. Animal models of Parkinson’s have evidenced iron loading inside the nucleoli of nigral neurons, however the nature of intranuclear iron deposition in the melanised neurons of the human substantia nigra is not understood. Here, scanning transmission x-ray microscopy (STXM) is used to probe iron foci in relation to the surrounding ultrastructure in melanised neurons of human substantia nigra from a confirmed Parkinson’s case. In addition to the expected neuromelanin-bound iron, iron deposits are also associated with the edge of the cell nucleolus. Speciation analysis confirms these deposits to be ferric (Fe3+) iron. The function of intranuclear iron in these cells remains unresolved, although both damaging and protective mechanisms are considered. This finding shows that STXM is a powerful label-free tool for the in situ, nanoscale chemical characterisation of both organic and inorganic intracellular components. Future applications are likely to shed new light on incompletely understood biochemical mechanisms, such as metal dysregulation and morphological changes to cell nucleoli, that are important in understanding the pathogenesis of Parkinson’s.

Citation

Brooks, J., Everett, J., Hill, E., Billimoria, K., Morris, C. M., Sadler, P. J., …Collingwood, J. F. (in press). Nanoscale synchrotron x-ray analysis of intranuclear iron in melanised neurons of Parkinson’s substantia nigra. Communications Biology, 7(1), Article 1024. https://doi.org/10.1038/s42003-024-06636-1

• Journal Article Type: Article
• Acceptance Date: Jul 26, 2024
• Online Publication Date: Aug 20, 2024
• Deposit Date: Aug 27, 2024
• Journal: Communications Biology
• Print ISSN: 2399-3642
• Electronic ISSN: 2399-3642
• Publisher: Nature Research
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 1
• Article Number: 1024
• DOI: https://doi.org/10.1038/s42003-024-06636-1
• Keywords: Analytical biochemistry; Iron; Microscopy; Neurochemistry; Parkinson's disease
• Public URL: https://keele-repository.worktribe.com/output/888141
• Publisher URL: https://www.nature.com/articles/s42003-024-06636-1