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DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS (2015)
Journal Article
Glossop, J. R., Haworth, K. E., Emes, R. D., Nixon, N. B., Packham, J. C., Dawes, P. T., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2015). DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7(4), 539 -551. https://doi.org/10.2217/epi.15.15

AIM: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). We profiled genome-wide DNA methylation in these cells from synovial fluid, a more readily ac... Read More about DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS.

O51. DNA Methylation Profiling of Synovial Fluid-Derived Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis Reveals Common and Distinct Changes Relative to their Tissue-Derived Counterparts (2015)
Presentation / Conference Contribution
Glossop, J. R., Haworth, K. E., Emes, R. D., Nixon, N. B., Packham, J. C., Dawes, P. T., Fryer, A. A., Mattey, D. L., & Farrell, W. E. O51. DNA Methylation Profiling of Synovial Fluid-Derived Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis Reveals Common and Distinct Changes Relative to their Tissue-Derived Counterparts

Background: Fibroblast-like synoviocytes (FLS) from patients with RA display an aggressive, invasive phenotype and play a key role in joint destruction. Increasing evidence indicates that alterations to the epigenome, including DNA methylation, contr... Read More about O51. DNA Methylation Profiling of Synovial Fluid-Derived Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis Reveals Common and Distinct Changes Relative to their Tissue-Derived Counterparts.

Revisiting screening for type 2 diabetes mellitus: the case for and against using HbA1c (2015)
Journal Article
Hanna, F. W. F., Wilkie, V., Issa, B. G., & Fryer, A. A. (2015). Revisiting screening for type 2 diabetes mellitus: the case for and against using HbA1c. British Journal of General Practice (BJGP), 65(633), E278-E280. https://doi.org/10.3399/bjgp15X684637

Type 2 diabetes mellitus is a major cause of morbidity and premature mortality in the Western world. It is responsible for about 10% of the NHS spend (about £286/second in the UK). Of those diagnosed with type 2 diabetes, 25% are thought already to h... Read More about Revisiting screening for type 2 diabetes mellitus: the case for and against using HbA1c.

Timing of HbA1c pre-admission: impact on readmission and in-hospital mortality in patients with diabetes (2015)
Presentation / Conference
Arora, A., Shanmugasundaram, M., Varughese, G., Duffs, C., Fryer, A., & Nayak, A. (2015, March). Timing of HbA1c pre-admission: impact on readmission and in-hospital mortality in patients with diabetes. Poster presented at Diabetes UK Professional Conference 2015, ExCel London, UK

Aims: Association between high HbA1c and risk of all-cause hospital admissions is recognised. We aimed to investigate the impact of HbA1c screening pre-admission the time of admission, on readmission rates and in-hospital mortality.
Methods: Amongst... Read More about Timing of HbA1c pre-admission: impact on readmission and in-hospital mortality in patients with diabetes.

Methylation of the FGFR2 gene is associated with high birth weight centile in humans (2014)
Journal Article
Haworth, K. E., Farrell, W. E., Emes, R. D., Ismail, K. M. K., Carroll, W. D., Hubball, E., Rooney, A., Yates, A. M., Mein, C., & Fryer, A. A. (2014). Methylation of the FGFR2 gene is associated with high birth weight centile in humans. Epigenomics, 6(5), 477-491. https://doi.org/10.2217/EPI.14.40

Aims: This study examined links between DNA methylation and birth weight centile (BWC), and explored the impact of genetic variation. Materials & methods: Using HumanMethylation450 arrays, we examined candidate gene-associated CpGs in cord blood from... Read More about Methylation of the FGFR2 gene is associated with high birth weight centile in humans.

Measuring vitamin D levels: surrogates are insufficient (2014)
Journal Article
Heald, A. H., Anderson, S. G., Scargill, J. J., Short, A. D., Holland, D., Livingston, M., Fryer, A. A., & Donn, R. P. (2015). Measuring vitamin D levels: surrogates are insufficient. International Journal of Clinical Practice, 69(1), 131-135. https://doi.org/10.1111/ijcp.12505

With the increasing evidence of adverse consequences because of low vitamin D levels on health demand for vitamin D, screening is increasing.

The objective of the study was to assess whether parathyroid hormone (PTH) levels/bone profile is suffici... Read More about Measuring vitamin D levels: surrogates are insufficient.

Reduced Testing Frequency for Glycated Hemoglobin, HbA(1c), Is Associated With Deteriorating Diabetes Control (2014)
Journal Article
Driskell, O. J., Holland, D., Waldron, J. L., Ford, C., Scargill, J. J., Heald, A., Tran, M., Hanna, F. W., Jones, P. W., Pemberton, R. J., & Fryer, A. A. (2014). Reduced Testing Frequency for Glycated Hemoglobin, HbA(1c), Is Associated With Deteriorating Diabetes Control. Diabetes Care, 37(10), 2731-2737. https://doi.org/10.2337/dc14-0297

Objective: We previously showed that in patients with diabetes mellitus, glycated hemoglobin (HbA1c) monitoring outside international guidance on testing frequency is widespread. Here we examined the relationship between testing frequency and diabete... Read More about Reduced Testing Frequency for Glycated Hemoglobin, HbA(1c), Is Associated With Deteriorating Diabetes Control.

Genome-wide DNA methylation profiling in rheumatoid arthritis identifies disease-associated methylation changes that are distinct to individual T- and B-lymphocyte populations (2014)
Journal Article
Glossop, J. R., Emes, R. D., Nixon, N. B., Haworth, K. E., Packham, J. C., Dawes, P. T., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2014). Genome-wide DNA methylation profiling in rheumatoid arthritis identifies disease-associated methylation changes that are distinct to individual T- and B-lymphocyte populations. Epigenetics, 9(9), 1228-1237. https://doi.org/10.4161/epi.29718

Changes to the DNA methylome have been described in patients with rheumatoid arthritis (RA). In previous work, we reported genome-wide methylation differences in T-lymphocyte and B-lymphocyte populations from healthy individuals. Now, using HumanMeth... Read More about Genome-wide DNA methylation profiling in rheumatoid arthritis identifies disease-associated methylation changes that are distinct to individual T- and B-lymphocyte populations.

Daylight robbery: Hospitals admissions for childhood asthma in English regions are inversely associated with hours of sunshine (2014)
Presentation / Conference
Carroll, W., Saunders, A., Ismail, K., Farrell, W., Emes, R., & Fryer, A. (2014, September). Daylight robbery: Hospitals admissions for childhood asthma in English regions are inversely associated with hours of sunshine. Presented at 22nd Annual Congress, Munich, Germany

Background: Vitamin D is implicated in the expression of childhood asthma but it is difficult to pick apart cause and effect from observational data. If lack of vitamin D is secondary to a sedentary lifestyle then weather patterns should not effect a... Read More about Daylight robbery: Hospitals admissions for childhood asthma in English regions are inversely associated with hours of sunshine.

Evaluation of accuracy of mediolateral episiotomy incisions using a training model (2014)
Journal Article
Silf, K., Woodhead, N., Kelly, J., Fryer, A., Kettle, C., & Ismail, K. M. K. (2015). Evaluation of accuracy of mediolateral episiotomy incisions using a training model. Midwifery, 31(1), 197-200. https://doi.org/10.1016/j.midw.2014.08.009

Background: Episiotomy is one of the most commonly performed surgical procedures worldwide. In the UK the use of episiotomy is selective, rather than routine, and a right mediolateral episiotomy (RMLE) is considered standard practice. According to Th... Read More about Evaluation of accuracy of mediolateral episiotomy incisions using a training model.

263. Genome-Wide DNA Methylation Profiling Identifies Distinct Changes in T- and B-Cell Populations from Patients with Rheumatoid Arthritis (2014)
Presentation / Conference Contribution
Glossop, J. R., Nixon, N. B., Emes, R. D., Haworth, K. E., Packham, J. C., Dawes, P. T., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2014, April). 263. Genome-Wide DNA Methylation Profiling Identifies Distinct Changes in T- and B-Cell Populations from Patients with Rheumatoid Arthritis. Paper presented at Rheumatology 2014, Liverpool, UK

Background: DNA methylation is one of several epigenetic mechanisms that impacts on gene expression without underlying changes to the DNA sequence. Recent studies describe changes to the DNA methylome in RA. To date however, no systematic genome-wide... Read More about 263. Genome-Wide DNA Methylation Profiling Identifies Distinct Changes in T- and B-Cell Populations from Patients with Rheumatoid Arthritis.

Genome-Wide Profiling Identifies Significant Differences Between The T-Lymphocyte and B-Lymphocyte Methylomes In Healthy Individuals (2013)
Presentation / Conference Contribution
Glossop, J., Nixon, N., Emes, R., Haworth, K., Packham, J., Dawes, P., Fryer, A., Mattey, D., & Farrell, W. (2013, October). Genome-Wide Profiling Identifies Significant Differences Between The T-Lymphocyte and B-Lymphocyte Methylomes In Healthy Individuals. Paper presented at 2013 ACR/ARHP Annual Meeting, San Diego, CA, USA

Epigenome-wide profiling identifies significant differences in DNA methylation between matched-pairs of T-and B-lymphocytes from healthy individuals (2013)
Journal Article
Glossop, J. R., Nixon, N. B., Emes, R. D., Haworth, K. E., Packham, J. C., Dawes, P. T., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2013). Epigenome-wide profiling identifies significant differences in DNA methylation between matched-pairs of T-and B-lymphocytes from healthy individuals. Epigenetics, 8(11), 1188-1197. https://doi.org/10.4161/epi.26265

Multiple reports now describe changes to the DNA methylome in rheumatoid arthritis and in many cases have analyzed methylation in mixed cell populations from whole blood. However, these approaches may preclude the identification of cell type-specific... Read More about Epigenome-wide profiling identifies significant differences in DNA methylation between matched-pairs of T-and B-lymphocytes from healthy individuals.

Combined influence of gene-specific cord blood methylation and maternal smoking habit on birth weight (2013)
Journal Article
Haworth, K. E., Farrell, W. E., Emes, R. D., Ismail, K. M. K., Carroll, W. D., Borthwick, H.-A. D., Yates, A. M., Hubball, E., Rooney, A., Khanam, M., Aggarwal, N., Jones, P. W., & Fryer, A. A. (2013). Combined influence of gene-specific cord blood methylation and maternal smoking habit on birth weight. Epigenomics, 5(1), 37-49. https://doi.org/10.2217/EPI.12.72

Aim: Evidence suggests that folic acid intake affects birth weight and that these effects may be mediated via the fetal epigenome. Our previous array data indicate that methylation in human cord blood at gene-specific CpGs is associated with birth we... Read More about Combined influence of gene-specific cord blood methylation and maternal smoking habit on birth weight.

Antiepileptic drugs and the fetal epigenome (2012)
Journal Article
Emes, R. D., Clifford, H., Haworth, K. E., Farrell, W. E., Fryer, A. A., Carroll, W. D., Ismail, K. M. K., & KM, I. (2013). Antiepileptic drugs and the fetal epigenome. Epilepsia, 54(1), e16-e19. https://doi.org/10.1111/j.1528-1167.2012.03673.x

Antiepileptic drugs (AEDs) can lower maternal folate and increase maternal homocysteine levels, which are known to affect the methyl cycle and hence DNA methylation levels. The influence of in utero exposure to AEDs on fetal DNA methylation was inves... Read More about Antiepileptic drugs and the fetal epigenome.

Managing demand for laboratory tests: a laboratory toolkit (2012)
Journal Article
Fryer, A. A., & Smellie, W. S. A. (2013). Managing demand for laboratory tests: a laboratory toolkit. Journal of Clinical Pathology, 66(1), 62-72. https://doi.org/10.1136/jclinpath-2011-200524

Healthcare budgets worldwide are facing increasing pressure to reduce costs and improve efficiency, while maintaining quality. Laboratory testing has not escaped this pressure, particularly since pathology investigations cost the National Health Serv... Read More about Managing demand for laboratory tests: a laboratory toolkit.

Limitations of glycosylated haemoglobin (HbA1c) in diabetes screening (2012)
Journal Article
WF Hanna, F., Geen, J., Issa, B. G., Tahrani, A. A., & Fryer, A. A. (2012). Limitations of glycosylated haemoglobin (HbA1c) in diabetes screening. Practical Diabetes, 29(1), 29-31. https://doi.org/10.1002/pdi.1653

Recently, glycosylated haemoglobin (HbA1c) has been recommended by the American Diabetes Association (ADA), the World Health Organisation and subsequently by many other professional bodies as a diagnostic tool for diabetes mellitus. However, the cut-... Read More about Limitations of glycosylated haemoglobin (HbA1c) in diabetes screening.

Vitamin D and oestrogen receptor polymorphisms in developmental dysplasia of the hip and primary protrusio acetabuli – A preliminary study (2007)
Journal Article
Kapoor, B., Dunlop, C., Wynn-Jones, C., Fryer, A. A., Strange, R. C., & Maffulli, N. (2007). Vitamin D and oestrogen receptor polymorphisms in developmental dysplasia of the hip and primary protrusio acetabuli – A preliminary study. Journal of Negative Results in BioMedicine, 6, Article 7. https://doi.org/10.1186/1477-5751-6-7

We investigated the association of developmental dysplasia of the hip (DDH) and primary protrusion acetabuli (PPA) with Vitamin D receptor polymorphisms Taq I and Fok I and oestrogen receptor polymorphisms Pvu II and Xba I. 45 patients with DDH and 2... Read More about Vitamin D and oestrogen receptor polymorphisms in developmental dysplasia of the hip and primary protrusio acetabuli – A preliminary study.