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All Outputs (39)

Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model (2024)
Journal Article
Perry, J., Mennan, C., Cool, P., McCarthy, H. S., Newell, K., Hopkins, T., …Roberts, S. (2024). Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model. Cartilage, https://doi.org/10.1177/19476035241287832

Objective
To determine if mesenchymal stromal cells (MSCs) derived from human umbilical cords (hUC) could reduce degeneration developing when injected into the knee of a large animal model of osteoarthritis (OA).
Design
Ten million culture-expande... Read More about Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model.

V2 Ovine UC-MSCs Revised Manuscript (2024)
Journal Article
Perry, J., Mennan, C., Cool, P., McCarthy, H., Newell, K., Hopkins, T., …Roberts, S. (in press). V2 Ovine UC-MSCs Revised Manuscript. Cartilage,

Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study (2024)
Journal Article
Wilkinson, H., McDonald, J., McCarthy, H., Perry, J., Wright, K., Hulme, C., & Cool, P. (in press). Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study. Briefings in Functional Genomics, Article elae008. https://doi.org/10.1093/bfgp/elae008

This project investigates if third-generation genomic sequencing can be used to identify the species of bacteria causing prosthetic joint infections (PJIs) at the time of revision surgery. Samples of prosthetic fluid were taken during revision surger... Read More about Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study.

CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS (2023)
Journal Article
Lan, T., Wright, K., Makwana, N., Bing, A., McCarthy, H., & Hulme, C. (2023). CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS. Orthopaedic Proceedings, 105-B(SUPP_16), 62-62. https://doi.org/10.1302/1358-992x.2023.16.062

AbstractObjectivesBone marrow aspirate concentrate (BMAC), together with fibrin glue (Tisseel, Baxter, UK) and Hyaluronic acid (HA) were used as a one-step cell therapy treating patients with ankle cartilage defects in our hospital. This therapy was... Read More about CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS.

SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS (2023)
Journal Article
Wright, K., McDonald, J., Mennan, C., Perry, J., Peffers, M., & Hulme, C. (2023). SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS. Orthopaedic Proceedings, 105-B(SUPP_16), 53-53. https://doi.org/10.1302/1358-992x.2023.16.053

AbstractObjectivesA promising therapy for early osteoarthritis (OA) is the transplantation of human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). The synovial fluid (SF) from a pre-clinical ovine model treated with hUC-MSCs has been pr... Read More about SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS.

A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications (2023)
Journal Article
Hulme, C. H., Mennan, C., McCarthy, H. S., Davies, R., Lan, T., Rix, L., …Wright, K. (2023). A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications. Cytotherapy, 25(10), 1017-1026. https://doi.org/10.1016/j.jcyt.2023.04.004

The Quantum cell expansion system manufactured by Terumo-BCT is perhaps the most widely reported Good Manufacturing Practice–compliant bioreactor used for the expansion of adherent cell populations, both for research purposes and cl... Read More about A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications.

The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies (2023)
Journal Article
Hulme, C. H., Garcia, J., Mennan, C., Perry, J., Roberts, S., Norris, K., …Wright, K. T. (2023). The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies. Tissue Engineering Part C: Methods, https://doi.org/10.1089/ten.tec.2023.0037

Background: Allogeneic chondrocyte therapies need to be developed to allow more individuals to be treated with a cell therapy for cartilage repair and to reduce the burden and cost of current two-stage autologous procedures. Up-scale manufacture of c... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.

PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA (2023)
Journal Article
Hulme, C., Roberts, S., Gallagher, P., Jermin, P., & Wright, K. (2023). PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA. Orthopaedic Proceedings, 105-B(SUPP_9), 76-76. https://doi.org/10.1302/1358-992x.2023.9.076

Stratification is required to ensure that only those patients likely to benefit, receive Autologous Chondrocyte Implantation (ACI); ideally by assessing a biomarker in the blood. This study aimed to assess differences in the plasma proteome of indivi... Read More about PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA.

Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening. (2023)
Journal Article
Hulme, C., & Wright, K. (2023). Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening. American Journal of Sports Medicine, 51(6), 1422-1433. https://doi.org/10.1177/03635465231156616

BACKGROUND: Stratification is required to ensure that only patients likely to benefit receive autologous chondrocyte implantation (ACI). It would be advantageous to identify biomarkers to predict ACI outcome that are measurable in blood, avoiding the... Read More about Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening..

A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome (2022)
Journal Article
Harrington, G. M. B., Cool, P., Hulme, C., Osman, A., Chowdhury, J. R., Kumar, N., …Wright, K. (2022). A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome. arXiv, https://doi.org/10.1101/2022.07.12.499696

<jats:sec><jats:label>1</jats:label><jats:title>Abstract</jats:title><jats:sec><jats:label>1.1</jats:label><jats:title>Introduction</jats:title><jats:p>Spinal Cord Injury (SCI) is a major cause of disability, with complications post-injury often lead... Read More about A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome.

Investigation of the blood proteome in response to spinal cord injury in rodent models (2021)
Journal Article
Hulme, C. H., Fuller, H. R., Riddell, J., Shirran, S. L., Botting, C. H., Osman, A., & Wright, K. T. (2022). Investigation of the blood proteome in response to spinal cord injury in rodent models. Spinal Cord, 60(4), 320-325. https://doi.org/10.1038/s41393-021-00692-8

STUDY DESIGN: Explanatory and Mechanistic Study
OBJECTIVES: A better understanding of the ‘whole-body’ response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying... Read More about Investigation of the blood proteome in response to spinal cord injury in rodent models.

Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy (2021)
Journal Article
Hulme, & Wright Bevans. (2021). Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy. American Journal of Sports Medicine, 1512-1523

Background: Biomarkers are needed to predict clinical outcome for microfracture and osteotomy surgeries to ensure patients can be better stratified to receive the most appropriate treatment.
Hypothesis/purpose: To identify novel biomarker candidate... Read More about Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy.

The Management of Talar Osteochondral Lesions - Current Concepts (2021)
Journal Article
Lan, T., McCarthy, H., Hulme, C., Wright, K., & Makwana, N. (2021). The Management of Talar Osteochondral Lesions - Current Concepts. Journal of Arthroscopy and Joint Surgery, 231-237. https://doi.org/10.1016/j.jajs.2021.04.002

Osteochondral lesions of the talus (OLTs) are a common complication following trauma, involving both the articular cartilage and the underlying subchondral bone, with variable aetiologies and often presenting with non-specific symptoms. Diagnosis of... Read More about The Management of Talar Osteochondral Lesions - Current Concepts.

Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy (2021)
Journal Article
Hulme, C. H., Peffers, M. J., Harrington, G. M. B., Wilson, E., Perry, J., Roberts, S., …Wright, K. T. (2021). Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy. American Journal of Sports Medicine, 49(6), 1512-1523. https://doi.org/10.1177/0363546521995565

Background:
Biomarkers are needed to predict clinical outcomes for microfracture and osteotomy surgeries to ensure patients can be better stratified to receive the most appropriate treatment.
Purpose:
To identify novel biomarker candidates and to... Read More about Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy.

Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy (2021)
Journal Article
Hulme, & Wright, K. (2021). Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy. Journal of Bone and Joint Surgery, British Volume, https://doi.org/10.1302/1358-992X.2021.2.008

Objectives
The ability to predict which patients will improve following routine surgeries aimed at preventing the progression of osteoarthritis is needed to aid patients being stratified to receive the most appropriate treatment. This study aimed to... Read More about Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy.

Evaluating patient perspectives on participating in scientific research and clinical trials for the treatment of spinal cord injury (2021)
Journal Article
Bahsoun, S., Kuiper, J., Hulme, C., Twigg, A., El Masri, W., Glass, C., …Wright, K. (2021). Evaluating patient perspectives on participating in scientific research and clinical trials for the treatment of spinal cord injury. Scientific reports, 11, Article 4361. https://doi.org/10.1038/s41598-021-83211-2

A questionnaire was developed to evaluate patients' perspective on research aimed at improving functions and overcoming complications associated with spinal cord injury (SCI). The first three sections were based on published and validated assessment... Read More about Evaluating patient perspectives on participating in scientific research and clinical trials for the treatment of spinal cord injury.

Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury (2020)
Journal Article
Harrington, G. M. B., Cool, P., Hulme, C., Osman, A., Roy Chowdhury, J., Kumar, N., …Wright, K. (2020). Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury. Journal of Neurotrauma, 38(3), https://doi.org/10.1089/neu.2020.7144

Neurological outcomes following spinal cord injury (SCI) are currently difficult to predict. Whilst the initial American Spinal Injury Association (ASIA) Impairment Scale (AIS) grade can give an estimate of outcome, the high remaining degree of uncer... Read More about Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury.

The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes (2020)
Journal Article
Mennan, Hulme, & Wright, K. (2020). The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes. Osteoarthritis and Cartilage Open, 1-8. https://doi.org/10.1016/j.ocarto.2020.100039

Objective The synovial fluid (SF) of patients with focal cartilage defects contains a population of poorly characterised cells that could have pathophysiological implications in early osteoarthritis and joint tissue repair. We have examined the cells... Read More about The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes.