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Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study (2024)
Journal Article
Wilkinson, H., McDonald, J., McCarthy, H., Perry, J., Wright, K., Hulme, C., & Cool, P. (in press). Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study. Briefings in Functional Genomics, Article elae008. https://doi.org/10.1093/bfgp/elae008

This project investigates if third-generation genomic sequencing can be used to identify the species of bacteria causing prosthetic joint infections (PJIs) at the time of revision surgery. Samples of prosthetic fluid were taken during revision surger... Read More about Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study.

The clinical potential of meniscal progenitor cells (2024)
Journal Article
Yan, W., Wang, J., Fan, P., Roberts, S., Wright, K., & Zhang, Z. (2024). The clinical potential of meniscal progenitor cells. Journal of Cartilage & Joint Preservation (JCJP), 100166. https://doi.org/10.1016/j.jcjp.2024.100166

Introduction The meniscus is an important cushioning structure of the knee joint, the maintenance of its normal structure and function plays a crucial role in protecting the joint from early degeneration. Stem/pro... Read More about The clinical potential of meniscal progenitor cells.

SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS (2023)
Journal Article
Wright, K., McDonald, J., Mennan, C., Perry, J., Peffers, M., & Hulme, C. (2023). SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS. Orthopaedic Proceedings, 105-B(SUPP_16), 53-53. https://doi.org/10.1302/1358-992x.2023.16.053

AbstractObjectivesA promising therapy for early osteoarthritis (OA) is the transplantation of human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). The synovial fluid (SF) from a pre-clinical ovine model treated with hUC-MSCs has been pr... Read More about SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS.

EVALUATING THE UTILITY OF SPECT/CT FOR THE IDENTIFICATION OF CARTILAGE LESIONS: A SYSTEMATIC REVIEW (2023)
Journal Article
Rix, L., Tushingham, S., Wright, K., & Snow, M. (2023). EVALUATING THE UTILITY OF SPECT/CT FOR THE IDENTIFICATION OF CARTILAGE LESIONS: A SYSTEMATIC REVIEW. Orthopaedic Proceedings, 105-B(SUPP_16), 45-45. https://doi.org/10.1302/1358-992x.2023.16.045

AbstractObjectiveA common orthopaedic pain found in a wide spectrum of individuals, from young and active to the elderly is anterior knee pain (AKP). It is a multifactorial disorder which is thought to occur through muscular imbalance, overuse, traum... Read More about EVALUATING THE UTILITY OF SPECT/CT FOR THE IDENTIFICATION OF CARTILAGE LESIONS: A SYSTEMATIC REVIEW.

CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS (2023)
Journal Article
Lan, T., Wright, K., Makwana, N., Bing, A., McCarthy, H., & Hulme, C. (2023). CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS. Orthopaedic Proceedings, 105-B(SUPP_16), 62-62. https://doi.org/10.1302/1358-992x.2023.16.062

AbstractObjectivesBone marrow aspirate concentrate (BMAC), together with fibrin glue (Tisseel, Baxter, UK) and Hyaluronic acid (HA) were used as a one-step cell therapy treating patients with ankle cartilage defects in our hospital. This therapy was... Read More about CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS.

Human vascularised synovium-on-a-chip: a mechanically stimulated, microfluidic model to investigate synovial inflammation and monocyte recruitment. (2023)
Journal Article
Thompson, C. L., Hopkins, T., Bevan, C., Screen, H. R., Wright, K. T., & Knight, M. M. (in press). Human vascularised synovium-on-a-chip: a mechanically stimulated, microfluidic model to investigate synovial inflammation and monocyte recruitment. Biomedical Materials, 18(6), https://doi.org/10.1088/1748-605X/acf976

Healthy synovium is critical for joint homeostasis. Synovial inflammation (synovitis) is implicated in the onset, progression and symptomatic presentation of arthritic joint diseases such as rheumatoid arthritis (RA) and osteoarthritis (OA). Thus, th... Read More about Human vascularised synovium-on-a-chip: a mechanically stimulated, microfluidic model to investigate synovial inflammation and monocyte recruitment..

The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies (2023)
Journal Article
Hulme, C. H., Garcia, J., Mennan, C., Perry, J., Roberts, S., Norris, K., …Wright, K. T. (2023). The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies. Tissue Engineering Part C: Methods, https://doi.org/10.1089/ten.tec.2023.0037

Background: Allogeneic chondrocyte therapies need to be developed to allow more individuals to be treated with a cell therapy for cartilage repair and to reduce the burden and cost of current two-stage autologous procedures. Up-scale manufacture of c... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.

Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release (2023)
Journal Article
Davies, R., Allen, S., Mennan, C., Platt, M., Wright, K., & Kehoe, O. (2023). Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release. International Journal of Molecular Sciences, 24(11), 9242. https://doi.org/10.3390/ijms24119242

The immunomodulatory properties of MSCs can be recreated using their extracellular vesicles (EVs). Yet, the true capabilities of the MSC EVs cannot be distinguished from contaminating bovine EVs and protein derived from supplemental foetal bovine ser... Read More about Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release.

Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report (2023)
Journal Article
Wilkinson, H., McCarthy, H. S., Perry, J., Smith, T., Wright, K., & Cool, P. (2023). Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report. Cureus, 15(5), https://doi.org/10.7759/cureus.38788

There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial... Read More about Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report.

A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications (2023)
Journal Article
Hulme, C. H., Mennan, C., McCarthy, H. S., Davies, R., Lan, T., Rix, L., …Wright, K. (2023). A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications. Cytotherapy, 25(10), 1017-1026. https://doi.org/10.1016/j.jcyt.2023.04.004

The Quantum cell expansion system manufactured by Terumo-BCT is perhaps the most widely reported Good Manufacturing Practice–compliant bioreactor used for the expansion of adherent cell populations, both for research purposes and cl... Read More about A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications.

PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA (2023)
Journal Article
Hulme, C., Roberts, S., Gallagher, P., Jermin, P., & Wright, K. (2023). PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA. Orthopaedic Proceedings, 105-B(SUPP_9), 76-76. https://doi.org/10.1302/1358-992x.2023.9.076

Stratification is required to ensure that only those patients likely to benefit, receive Autologous Chondrocyte Implantation (ACI); ideally by assessing a biomarker in the blood. This study aimed to assess differences in the plasma proteome of indivi... Read More about PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA.

Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening. (2023)
Journal Article
Hulme, C., & Wright, K. (2023). Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening. American Journal of Sports Medicine, 51(6), 1422-1433. https://doi.org/10.1177/03635465231156616

BACKGROUND: Stratification is required to ensure that only patients likely to benefit receive autologous chondrocyte implantation (ACI). It would be advantageous to identify biomarkers to predict ACI outcome that are measurable in blood, avoiding the... Read More about Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening..

A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome (2022)
Journal Article
Harrington, G. M. B., Cool, P., Hulme, C., Osman, A., Chowdhury, J. R., Kumar, N., …Wright, K. (2022). A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome. arXiv, https://doi.org/10.1101/2022.07.12.499696

1Abstract1.1IntroductionSpinal Cord Injury (SCI) is a major cause of disability, with complications post-injury often leading to life-long health issues with need of extensive treatment. Neurological outcome post-SCI can be variable and difficult to... Read More about A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome.

Investigation of the blood proteome in response to spinal cord injury in rodent models (2021)
Journal Article
Hulme, C. H., Fuller, H. R., Riddell, J., Shirran, S. L., Botting, C. H., Osman, A., & Wright, K. T. (2022). Investigation of the blood proteome in response to spinal cord injury in rodent models. Spinal Cord, 60(4), 320-325. https://doi.org/10.1038/s41393-021-00692-8

STUDY DESIGN: Explanatory and Mechanistic Study OBJECTIVES: A better understanding of the ‘whole-body’ response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying... Read More about Investigation of the blood proteome in response to spinal cord injury in rodent models.

Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model. (2021)
Journal Article
McCarthy, H., Perry, J., Mennan, C., & Wright, K. (2021). Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model. Cells, 1-16. https://doi.org/10.3390/cells10081999

Human umbilical cord (hUC)- or bone marrow (hBM)-derived mesenchymal stromal cells (MSCs) were evaluated as an allogeneic source of cells for cartilage repair. We aimed to determine if they could enhance healing of chondral defects with or without th... Read More about Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model..

An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans. (2021)
Journal Article
Wright, K., Roberts, S., & Kuiper, J. (2021). An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans. Cells, https://doi.org/10.3390/cells10081903

Chondrocyte-based cartilage repair strategies, such as articular chondrocyte implantation, are widely used, but few studies addressed the communication between native subchondral bone cells and the transplanted chondrocytes. An indirect co-culture mo... Read More about An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans..

The Management of Talar Osteochondral Lesions - Current Concepts (2021)
Journal Article
Lan, T., McCarthy, H., Hulme, C., Wright, K., & Makwana, N. (2021). The Management of Talar Osteochondral Lesions - Current Concepts. Journal of Arthroscopy and Joint Surgery, 231-237. https://doi.org/10.1016/j.jajs.2021.04.002

Osteochondral lesions of the talus (OLTs) are a common complication following trauma, involving both the articular cartilage and the underlying subchondral bone, with variable aetiologies and often presenting with non-specific symptoms. Diagnosis of... Read More about The Management of Talar Osteochondral Lesions - Current Concepts.

Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy (2021)
Journal Article
Hulme, C. H., Peffers, M. J., Harrington, G. M. B., Wilson, E., Perry, J., Roberts, S., …Wright, K. T. (2021). Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy. American Journal of Sports Medicine, 49(6), 1512-1523. https://doi.org/10.1177/0363546521995565

Background: Biomarkers are needed to predict clinical outcomes for microfracture and osteotomy surgeries to ensure patients can be better stratified to receive the most appropriate treatment. Purpose: To identify novel biomarker candidates and to... Read More about Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy.