All Outputs (10)

Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain. (2021)
Journal Article
Schuurs, Z. P., Hammond, E., Elli, S., Rudd, T. R., Mycroft-West, C. J., Lima, M. A., Skidmore, M. A., Karlsson, R., Chen, Y.-H., Bagdonaite, I., Yang, Z., Ahmed, Y. A., Richard, D. J., Turnbull, J., Ferro, V., Coombe, D. R., & Gandhi, N. S. (2021). Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain. Computational and Structural Biotechnology Journal, 19, 2806 - 2818. https://doi.org/10.1016/j.csbj.2021.05.002

SARS-CoV-2 has rapidly spread throughout the world's population since its initial discovery in 2019. The virus infects cells via a glycosylated spike protein located on its surface. The protein primarily binds to the angiotensin-converting enzyme-2 (... Read More about Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain..

Nebulized enriched heparin to treat no critical patients with Sars-Cov-2 (2021)
Journal Article
Bertanha, M., Rodrigues, L. D. S., Mellucci Filho, P. L., Moroz, A., Pardini, M. I. D. M. C., Sobreira, M. L., Durigon, E. L., Machado, R. R. G., Grotto, R. M. T., Andrade de Lima, M., Nader, H. B., Moraes, M. L. D., Barbosa, A. N., Medolago, N. B., Cardoso, F. F., Magro, A. J., Carvalho, C. R. G., Moraes, L. N. D., Alvarado, R. D. C., Nunes, H. C., …Fortaleza, C. M. C. B. (in press). Nebulized enriched heparin to treat no critical patients with Sars-Cov-2. Medicine, 100(51), e28288. https://doi.org/10.1097/md.0000000000028288

Background: Coronavirus disease 2019 (COVID-19) is a viral respiratory disease that spreads rapidly, reaching pandemic status, causing the collapse of numerous health systems, and a strong economic and social impact. The treatment so far has not been... Read More about Nebulized enriched heparin to treat no critical patients with Sars-Cov-2.

Chemical synthesis of 4′-thio and 4′-sulfinyl pyrimidine nucleoside analogues (2021)
Journal Article
Guinan, M., Huang, N., Hawes, C. S., Lima, M. A., Smith, M., & Miller, G. J. (2021). Chemical synthesis of 4′-thio and 4′-sulfinyl pyrimidine nucleoside analogues. Organic and Biomolecular Chemistry, 20(7), 1401-1406. https://doi.org/10.1039/d1ob02097h

Analogues of the canonical nucleosides required for nucleic acid synthesis have a longstanding presence and proven capability within antiviral and anticancer research. 4'-Thionucleosides, that incorporate bioisosteric replacement of furanose oxygen w... Read More about Chemical synthesis of 4′-thio and 4′-sulfinyl pyrimidine nucleoside analogues.

The Hyperlipidaemic Drug Fenofibrate Significantly Reduces Infection by SARS-CoV-2 in Cell Culture Models. (2021)
Journal Article
Davies, S., Mycroft-West, C., Pagani, I., Hill, H., Chen, Y.-H., Karlsson, R., Bagdonaite, I., Guimond, S., Stamataki, Z., Andrade De Lima, M., Turnbull, J., Yang, Z., Vicenzi, E., Skidmore, M., Khanim, F., & Richardson, A. (2021). The Hyperlipidaemic Drug Fenofibrate Significantly Reduces Infection by SARS-CoV-2 in Cell Culture Models. Frontiers in Pharmacology, 12, 660490. https://doi.org/10.3389/fphar.2021.660490

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has caused a significant number of fatalities and worldwide disruption. To identify drugs to repurpose to treat SARS-CoV-2 infections, we established a screen to measure the di... Read More about The Hyperlipidaemic Drug Fenofibrate Significantly Reduces Infection by SARS-CoV-2 in Cell Culture Models..

A new heparin fragment decreases liver ischemia-reperfusion injury (2021)
Journal Article
Vasques, E. R., Figueira, E. R., Rocha-Filho, J. A., Lanchotte, C., Ximenes, J. L., Nader, H. B., Tersariol, I. L., Lima, M. A., Rodrigues, T., Cunha, J. E., Chaib, E., D'Albuquerque, L. A., & Galvão, F. H. (2022). A new heparin fragment decreases liver ischemia-reperfusion injury. Hepatobiliary and Pancreatic Diseases International, 21(2), 190-192. https://doi.org/10.1016/j.hbpd.2021.07.004

Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β-Hairpin Antimicrobial Peptides (2021)
Journal Article
Buri, M. V., Sperandio, L. P., de Souza, K. F. S., Antunes, F., Rezende, M. M., Melo, C. M., Pinhal, M. A. S., Barros, C. C., Fernig, D. G., Yates, E. A., Ide, J. S., Smaili, S. S., Riske, K. A., Nader, H. B., Luis dos Santos Tersariol, I., Lima, M. A., Judice, W. A. S., Miranda, A., & Paredes-Gamero, E. J. (2021). Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β-Hairpin Antimicrobial Peptides. ACS Applied Biomaterials, 4(8), 6488--6501. https://doi.org/10.1021/acsabm.1c00390

The cytotoxic mode of action of four antimicrobial peptides (AMPs) (gomesin, tachyplesin, protegrin, and polyphemusin) against a HeLa cell tumor model is discussed. A study of cell death by AMP stimulation revealed some similarities, including annexi... Read More about Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β-Hairpin Antimicrobial Peptides.

Glycosaminoglycans from Litopenaeus vannamei Inhibit the Alzheimer’s Disease ß Secretase, BACE1 (2021)
Journal Article
Mycroft-West, C. J., Devlin, A. J., Cooper, L. C., Guimond, S. E., Procter, P., Guerrini, M., Miller, G. J., Fernig, D. G., Yates, E. A., Lima, M. A., & Skidmore, M. A. (2021). Glycosaminoglycans from Litopenaeus vannamei Inhibit the Alzheimer’s Disease ß Secretase, BACE1. Marine Drugs, 19(4), 203 -203. https://doi.org/10.3390/md19040203

Only palliative therapeutic options exist for the treatment of Alzheimer’s Disease; no new successful drug candidates have been developed in over 15 years. The widely used clinical anticoagulant heparin has been reported to exert beneficial effects t... Read More about Glycosaminoglycans from Litopenaeus vannamei Inhibit the Alzheimer’s Disease ß Secretase, BACE1.

Unfractionated heparin inhibits live wild type SARS-CoV-2 cell infectivity at therapeutically relevant concentrations. (2021)
Journal Article
Tree, J., Turnbull, J., Buttigieg, K., Elmore, M., Coombes, N., Hogwood, J., Mycroft-West, C., Andrade De Lima, M., Skidmore, M., Karlsson, R., Chen, Y.-H., Yang, Z., Spalluto, C., Staples, K., Yates, E., Gray, E., Singh, D., Wilkinson, T., Page, C., & Carroll, M. (2021). Unfractionated heparin inhibits live wild type SARS-CoV-2 cell infectivity at therapeutically relevant concentrations. British Journal of Pharmacology, 178, 626--635. https://doi.org/10.1111/bph.15304

BACKGROUND AND PURPOSE: Currently, there are no licensed vaccines and limited antivirals for the treatment of COVID-19. Heparin (delivered systemically) is currently used to treat anticoagulant anomalies in COVID-19 patients. Additionally, in the Uni... Read More about Unfractionated heparin inhibits live wild type SARS-CoV-2 cell infectivity at therapeutically relevant concentrations..

ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis (2021)
Journal Article
Meneghetti, M., Deboni, P., Palomino, C., Braga, L., Cavalheiro, R., Viana, G., Yates, E., Nader, H., & Andrade De Lima, M. (2021). ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. Carbohydrate Polymers, 117477--117477. https://doi.org/10.1016/j.carbpol.2020.117477

The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a... Read More about ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis.

Neuroprotective effect of heparin Trisulfated disaccharide on ischemic stroke (2021)
Journal Article
Chiarantin, G. M. D., Delgado-Garcia, L. M., Zamproni, L. N., Lima, M. A., Nader, H. B., Tersariol, I. L. S., & Porcionatto, M. (2021). Neuroprotective effect of heparin Trisulfated disaccharide on ischemic stroke. Glycoconjugate Journal, 38(1), 35--43. https://doi.org/10.1007/s10719-020-09966-4

Cells undergoing hypoxia experience intense cytoplasmic calcium (Ca2+) overload. High concentrations of intracellular calcium ([Ca2+]i) can trigger cell death in the neural tissue, a hallmark of stroke. Neural Ca2+ homeostasis involves regulation by... Read More about Neuroprotective effect of heparin Trisulfated disaccharide on ischemic stroke.