Skip to main content

Research Repository

Advanced Search

ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis

Meneghetti, MCZ; Deboni, P; Palomino, CMV; Braga, LP; Cavalheiro, RP; Viana, GM; Yates, EA; Nader, HB; Andrade De Lima, M

ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis Thumbnail


Authors

MCZ Meneghetti

P Deboni

CMV Palomino

LP Braga

RP Cavalheiro

GM Viana

EA Yates

HB Nader



Abstract

The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis.

Citation

Meneghetti, M., Deboni, P., Palomino, C., Braga, L., Cavalheiro, R., Viana, G., …Andrade De Lima, M. (2021). ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. Carbohydrate Polymers, 117477--117477. https://doi.org/10.1016/j.carbpol.2020.117477

Journal Article Type Article
Acceptance Date Nov 30, 2020
Publication Date 2021-01
Publicly Available Date May 26, 2023
Journal Carbohydrate Polymers
Print ISSN 0144-8617
Publisher Elsevier
Pages 117477--117477
DOI https://doi.org/10.1016/j.carbpol.2020.117477
Keywords Biosynthesis; Heparan sulfate; COPI; COPII; Golgi apparatus
Publisher URL https://www.sciencedirect.com/science/article/pii/S0144861720316507?via\%3Dihub

Files






You might also like



Downloadable Citations